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Article

Human Epidural AD–MSC Exosomes Improve Function Recovery after Spinal Cord Injury in Rats

1
Preclinical Research Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu 41061, Korea
2
Department of Biomaterials Science (BK21 Four Program), Pusan National University, Miryang 50463, Korea
3
Cellexobio, Co., Ltd., Daegu 42415, Korea
4
Department of Orthopedic Surgery, Yeungnam University Medical Center, Yeungnam University College of Medicine, 170, Hyochung-ro, Namgu, Daegu 42415, Korea
5
Company Culture Team, Kolmar Korea Holdings 61, Heolleung-ro 8 gill, Seocho-gu, Seoul 06800, Korea
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Pietro Veglianese
Biomedicines 2022, 10(3), 678; https://doi.org/10.3390/biomedicines10030678
Received: 10 December 2021 / Revised: 4 March 2022 / Accepted: 10 March 2022 / Published: 15 March 2022
(This article belongs to the Special Issue Spinal Cord Injury: From Mechanisms to Nanotherapeutic Approaches)
Spinal cord injury (SCI) interferes with the normal function of the autonomic nervous system by blocking circuits between the sensory and motor nerves. Although many studies focus on functional recovery after neurological injury, effective neuroregeneration is still being explored. Recently, extracellular vesicles such as exosomes have emerged as cell-free therapeutic agents owing to their ability of cell-to-cell communication. In particular, exosomes released from mesenchymal stem cells (MSCs) have the potential for tissue regeneration and exhibit therapeutic effectiveness in neurological disorders. In this study, we isolated exosomes from human epidural adipose tissue-derived MSCs (hEpi AD–MSCs) using the tangential flow filtration method. The isolated exosomes were analyzed for size, concentration, shape, and major surface markers using nanoparticle tracking analysis, transmission electron microscopy, and flow cytometry. To evaluate their effect on SCI recovery, hEpi AD–MSC exosomes were injected intravenously in SCI-induced rats. hEpi AD–MSC exosomes improved the locomotor function of SCI-induced rats. The results of histopathological and cytokine assays showed that hEpi AD–MSC exosomes regulated inflammatory response. Genetic profiling of the rat spinal cord tissues revealed changes in the expression of inflammation-related genes after exosome administration. Collectively, hEpi AD–MSC exosomes are effective in restoring spinal functions by reducing the inflammatory response. View Full-Text
Keywords: spinal cord injury; mesenchymal stem cells; exosomes; extracellular vesicles spinal cord injury; mesenchymal stem cells; exosomes; extracellular vesicles
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MDPI and ACS Style

Sung, S.-E.; Seo, M.-S.; Kim, Y.-I.; Kang, K.-K.; Choi, J.-H.; Lee, S.; Sung, M.; Yim, S.-G.; Lim, J.-H.; Seok, H.-G.; Yang, S.-Y.; Lee, G.-W. Human Epidural AD–MSC Exosomes Improve Function Recovery after Spinal Cord Injury in Rats. Biomedicines 2022, 10, 678. https://doi.org/10.3390/biomedicines10030678

AMA Style

Sung S-E, Seo M-S, Kim Y-I, Kang K-K, Choi J-H, Lee S, Sung M, Yim S-G, Lim J-H, Seok H-G, Yang S-Y, Lee G-W. Human Epidural AD–MSC Exosomes Improve Function Recovery after Spinal Cord Injury in Rats. Biomedicines. 2022; 10(3):678. https://doi.org/10.3390/biomedicines10030678

Chicago/Turabian Style

Sung, Soo-Eun, Min-Soo Seo, Young-In Kim, Kyung-Ku Kang, Joo-Hee Choi, Sijoon Lee, Minkyoung Sung, Sang-Gu Yim, Ju-Hyeon Lim, Hyun-Gyu Seok, Seung-Yun Yang, and Gun-Woo Lee. 2022. "Human Epidural AD–MSC Exosomes Improve Function Recovery after Spinal Cord Injury in Rats" Biomedicines 10, no. 3: 678. https://doi.org/10.3390/biomedicines10030678

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