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Article

The Interplay of Cx26, Cx32, Cx37, Cx40, Cx43, Cx45, and Panx1 in Inner-Ear Development of Yotari (dab1−/−) Mice and Humans

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Department of Anatomy, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and Herzegovina
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Department of Histology and Embryology, School of Medicine, University of Mostar, 88000 Mostar, Bosnia and Herzegovina
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Faculty of Health Studies, University of Mostar, 88000 Mostar, Bosnia and Herzegovina
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Mediterranean Institute for Life Sciences (MedILS), 21000 Split, Croatia
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Department of Anatomy, Histology and Embryology, School of Medicine, University of Split, Šoltanska 2, 21000 Split, Croatia
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Department of Anatomy, Shiga University of Medical Science, Otsu 520-2192, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Bernard Binetruy
Biomedicines 2022, 10(3), 589; https://doi.org/10.3390/biomedicines10030589
Received: 22 January 2022 / Revised: 28 February 2022 / Accepted: 1 March 2022 / Published: 3 March 2022
(This article belongs to the Special Issue Connexins and Pannexins in Embryonic and Fetal Development)
We investigated DAB1-protein deficiency in the inner-ear development of yotari in comparison to humans and wild-type (wt) mice by immunofluorescence for the expression of connexins (Cxs) and the pannexin Panx1. The spatial and temporal dynamics of Cx26, Cx32, Cx37, Cx40, Cx43, Cx45, and Panx1 were determined in the sixth and eighth weeks of human development and at the corresponding mouse embryonic E13.5 and E15.5, in order to examine gap junction intercellular communication (GJIC) and hemichannel formation. The quantification of the area percentage covered by positive signal was performed for the epithelium and mesenchyme of the cochlear and semicircular ducts and is expressed as the mean ± SD. The data were analysed by one-way ANOVA. Almost all of the examined Cxs were significantly decreased in the cochlear and semicircular ducts of yotari compared to wt and humans, except for Cx32, which was significantly higher in yotari. Cx40 dominated in human inner-ear development, while yotari and wt had decreased expression. The Panx1 expression in yotari was significantly lower than that in the wt and human inner ear, except at E13.5 in the mesenchyme of the wt and epithelium and mesenchyme of humans. Our results emphasize the relevance of GJIC during the development of vestibular and cochlear functions, where they can serve as potential therapeutic targets in inner-ear impairments. View Full-Text
Keywords: gap junction; inner-ear development; Cx26; Cx32; Cx37; Cx40; Cx43; Cx45; Panx1; yotari gap junction; inner-ear development; Cx26; Cx32; Cx37; Cx40; Cx43; Cx45; Panx1; yotari
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MDPI and ACS Style

Lesko, J.; Rastović, P.; Mišković, J.; Šoljić, V.; Paštar, V.; Zovko, Z.; Filipović, N.; Katsuyama, Y.; Saraga-Babić, M.; Vukojević, K. The Interplay of Cx26, Cx32, Cx37, Cx40, Cx43, Cx45, and Panx1 in Inner-Ear Development of Yotari (dab1−/−) Mice and Humans. Biomedicines 2022, 10, 589. https://doi.org/10.3390/biomedicines10030589

AMA Style

Lesko J, Rastović P, Mišković J, Šoljić V, Paštar V, Zovko Z, Filipović N, Katsuyama Y, Saraga-Babić M, Vukojević K. The Interplay of Cx26, Cx32, Cx37, Cx40, Cx43, Cx45, and Panx1 in Inner-Ear Development of Yotari (dab1−/−) Mice and Humans. Biomedicines. 2022; 10(3):589. https://doi.org/10.3390/biomedicines10030589

Chicago/Turabian Style

Lesko, Josip, Pejana Rastović, Josip Mišković, Violeta Šoljić, Vlatka Paštar, Zdenka Zovko, Natalija Filipović, Yu Katsuyama, Mirna Saraga-Babić, and Katarina Vukojević. 2022. "The Interplay of Cx26, Cx32, Cx37, Cx40, Cx43, Cx45, and Panx1 in Inner-Ear Development of Yotari (dab1−/−) Mice and Humans" Biomedicines 10, no. 3: 589. https://doi.org/10.3390/biomedicines10030589

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