Dynamic Interplay between Cockayne Syndrome Protein B and Poly(ADP-Ribose) Polymerase 1 during Oxidative DNA Damage Repair
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Menadione Treatment
2.2. Protein Fractionation and Western Blotting
2.3. shRNA Knockdown
2.4. Menadione Sensitivity Assays
2.5. ChIP-qPCR Analyses
2.6. Antibodies
3. Results
3.1. PARP1 Is Essential for CSB Recruitment to the Top Four Menadione-Induced CSB Binding Sites
3.2. CSB Regulates the Association of PARP1 with Chromatin upon Oxidative Stress
3.3. CSB Regulates PAR Levels on Chromatin in Oxidatively-Stressed Cells
3.4. CSB Facilitates the Recruitment of XRCC1 to Oxidatively Damaged Chromatin
3.5. Regulation of PARP1 Activity Is a Major CSB Function in Oxidatively-Stressed Cells
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lake, R.J.; Bilkis, R.; Fan, H.-Y. Dynamic Interplay between Cockayne Syndrome Protein B and Poly(ADP-Ribose) Polymerase 1 during Oxidative DNA Damage Repair. Biomedicines 2022, 10, 361. https://doi.org/10.3390/biomedicines10020361
Lake RJ, Bilkis R, Fan H-Y. Dynamic Interplay between Cockayne Syndrome Protein B and Poly(ADP-Ribose) Polymerase 1 during Oxidative DNA Damage Repair. Biomedicines. 2022; 10(2):361. https://doi.org/10.3390/biomedicines10020361
Chicago/Turabian StyleLake, Robert J., Rabeya Bilkis, and Hua-Ying Fan. 2022. "Dynamic Interplay between Cockayne Syndrome Protein B and Poly(ADP-Ribose) Polymerase 1 during Oxidative DNA Damage Repair" Biomedicines 10, no. 2: 361. https://doi.org/10.3390/biomedicines10020361