Synthetic Mono-Carbonyl Curcumin Analogues Attenuate Oxidative Stress in Mouse Models
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Animals
2.2. In Vitro Antioxidant Activity
2.3. Acute Toxicity Study
2.4. Experimental Design and Animal Dosing
2.5. Behavioral Studies
2.5.1. Light–Dark Box
2.5.2. Hole Board Test
2.5.3. Y-Maze Test
2.6. Assessment of Biochemical Parameters and Biomarker Study
2.6.1. Measurement of Malondialdehyde (MDA) Level
2.6.2. Catalase (CAT) Activity
2.6.3. Superoxide Dismutase (SOD) Activity
2.6.4. Measurement of Glutathione (GSH) Activity
2.7. Statistical Analysis
3. Results
3.1. In Vitro Antioxidant Activity
3.2. Acute Toxicity
3.3. In Vivo Behavioral Studies
3.3.1. Light–Dark Box Apparatus
3.3.2. Hole Board Assay
3.3.3. Y-Maze Test
3.4. Assessment of Biochemical Parameters and Biomarker Study
4. Discussion
5. Limitations
6. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compound | DPPH (IC50 µg/mL) | ABTS (IC50 µg/mL) |
---|---|---|
h1 | 160.32 ± 1.37 | 184.19 ± 1.37 |
h2 | 53.29 ± 2.13 | 70.21 ± 1.26 |
h3 | 82.43 ± 2.17 | 143.65 ± 2.43 |
h4 | 230.18 ± 2.15 | 356.38 ± 1.17 |
h5 | 357.13 ± 1.16 | 435.31 ± 1.18 |
Tocopherol | 9.16 ± 1.14 | 13.18 ± 1.16 |
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Hussain, H.; Ahmad, S.; Shah, S.W.A.; Ullah, A.; Rahman, S.U.; Ahmad, M.; Almehmadi, M.; Abdulaziz, O.; Allahyani, M.; Alsaiari, A.A.; et al. Synthetic Mono-Carbonyl Curcumin Analogues Attenuate Oxidative Stress in Mouse Models. Biomedicines 2022, 10, 2597. https://doi.org/10.3390/biomedicines10102597
Hussain H, Ahmad S, Shah SWA, Ullah A, Rahman SU, Ahmad M, Almehmadi M, Abdulaziz O, Allahyani M, Alsaiari AA, et al. Synthetic Mono-Carbonyl Curcumin Analogues Attenuate Oxidative Stress in Mouse Models. Biomedicines. 2022; 10(10):2597. https://doi.org/10.3390/biomedicines10102597
Chicago/Turabian StyleHussain, Haya, Shujaat Ahmad, Syed Wadood Ali Shah, Abid Ullah, Shafiq Ur Rahman, Manzoor Ahmad, Mazen Almehmadi, Osama Abdulaziz, Mamdouh Allahyani, Ahad Amer Alsaiari, and et al. 2022. "Synthetic Mono-Carbonyl Curcumin Analogues Attenuate Oxidative Stress in Mouse Models" Biomedicines 10, no. 10: 2597. https://doi.org/10.3390/biomedicines10102597