Anti-Inflammatory Activity of Panduratin A against LPS-Induced Microglial Activation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Panduratin A Material
2.2. Cell Culture
2.3. Cell Viability Assay
2.4. Nitric Oxide Assay
2.5. Reverse-Transcription Polymerase Chain Reaction
2.6. Enzyme-Linked Immunosorbent Assay
2.7. Western Blot
2.8. Statistical Analysis
3. Results
3.1. Cytotoxic Potency of Panduratin A in SIMA9 Cells
3.2. Panduratin A Suppressed Nitric Oxide Production, iNOS Expression, and NF-κB Activation
3.3. Panduratin A Reduced TNF-α, IL-1β, and IL-6 Production in LPS-Induced Microglial Activation
3.4. Panduratin A Enhanced IL-4 and IL-10 Production in LPS-Induced Microglial Activation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Jamornwan, S.; Chokpanuwat, T.; Uppakara, K.; Soodvilai, S.; Saengsawang, W. Anti-Inflammatory Activity of Panduratin A against LPS-Induced Microglial Activation. Biomedicines 2022, 10, 2587. https://doi.org/10.3390/biomedicines10102587
Jamornwan S, Chokpanuwat T, Uppakara K, Soodvilai S, Saengsawang W. Anti-Inflammatory Activity of Panduratin A against LPS-Induced Microglial Activation. Biomedicines. 2022; 10(10):2587. https://doi.org/10.3390/biomedicines10102587
Chicago/Turabian StyleJamornwan, Sopana, Tanida Chokpanuwat, Kwanchanok Uppakara, Sunhapas Soodvilai, and Witchuda Saengsawang. 2022. "Anti-Inflammatory Activity of Panduratin A against LPS-Induced Microglial Activation" Biomedicines 10, no. 10: 2587. https://doi.org/10.3390/biomedicines10102587