Model of Acute Liver Failure in an Isolated Perfused Porcine Liver—Challenges and Lessons Learned
Abstract
:1. Introduction
2. Materials and Methods
2.1. Liver Procurement
2.2. Normothermic Machine Perfusion (NMP)
2.3. Experimental Protocol
2.4. Perfusate Biochemistry
2.5. Histology
2.6. Chromogenic and Enzyme-Linked Immunosorbent Assays
2.7. In Vitro Testing of Acetaminophen Metabolites and CCl4
2.8. Statistical Analysis
3. Results
3.1. Perfusion Parameters
3.2. Perfusate Biochemistry
3.3. Histology
3.4. Measures of Oxidative Stress
3.5. Methemoglobinemia and Hemolysis
3.6. Carbon Tetrachloride (CCl4)
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Hefler, J.; Hatami, S.; Thiesen, A.; Olafson, C.; Durand, K.; Acker, J.; Karvellas, C.J.; Bigam, D.L.; Freed, D.H.; Shapiro, A.M.J. Model of Acute Liver Failure in an Isolated Perfused Porcine Liver—Challenges and Lessons Learned. Biomedicines 2022, 10, 2496. https://doi.org/10.3390/biomedicines10102496
Hefler J, Hatami S, Thiesen A, Olafson C, Durand K, Acker J, Karvellas CJ, Bigam DL, Freed DH, Shapiro AMJ. Model of Acute Liver Failure in an Isolated Perfused Porcine Liver—Challenges and Lessons Learned. Biomedicines. 2022; 10(10):2496. https://doi.org/10.3390/biomedicines10102496
Chicago/Turabian StyleHefler, Joshua, Sanaz Hatami, Aducio Thiesen, Carly Olafson, Kiarra Durand, Jason Acker, Constantine J. Karvellas, David L. Bigam, Darren H. Freed, and Andrew Mark James Shapiro. 2022. "Model of Acute Liver Failure in an Isolated Perfused Porcine Liver—Challenges and Lessons Learned" Biomedicines 10, no. 10: 2496. https://doi.org/10.3390/biomedicines10102496