Size Distribution of Inactivated Tick-Borne Encephalitis Virus Particles Revealed by a Comprehensive Physicochemical Approach
Abstract
:1. Introduction
2. Materials and Methods
2.1. Obtaining Inactivated Virions of the TBEV
2.2. Determination of the Hydrodynamic Size of Virions
2.3. Electron Microscopy
2.4. Spectroscopy of Characteristic Electron Energy Loss (EELS)
2.5. Cryo-Electron Microscopy
2.6. Atomic Force Microscopy
2.7. Image Processing
2.8. Cryo-EM Reconstruction of the Tick-Borne Encephalitis Virus
3. Results and Discussion
3.1. Virion Size
3.2. Analysis of TBEV Structure
3.3. Phosphorus Mapping via STEM-EELS
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Moiseenko, A.V.; Bagrov, D.V.; Vorovitch, M.F.; Uvarova, V.I.; Veselov, M.M.; Kashchenko, A.V.; Ivanova, A.L.; Osolodkin, D.I.; Egorov, A.M.; Ishmukhametov, A.A.; et al. Size Distribution of Inactivated Tick-Borne Encephalitis Virus Particles Revealed by a Comprehensive Physicochemical Approach. Biomedicines 2022, 10, 2478. https://doi.org/10.3390/biomedicines10102478
Moiseenko AV, Bagrov DV, Vorovitch MF, Uvarova VI, Veselov MM, Kashchenko AV, Ivanova AL, Osolodkin DI, Egorov AM, Ishmukhametov AA, et al. Size Distribution of Inactivated Tick-Borne Encephalitis Virus Particles Revealed by a Comprehensive Physicochemical Approach. Biomedicines. 2022; 10(10):2478. https://doi.org/10.3390/biomedicines10102478
Chicago/Turabian StyleMoiseenko, Andrey V., Dmitry V. Bagrov, Mikhail F. Vorovitch, Victoria I. Uvarova, Maxim M. Veselov, Anastasia V. Kashchenko, Alla L. Ivanova, Dmitry I. Osolodkin, Alexey M. Egorov, Aydar A. Ishmukhametov, and et al. 2022. "Size Distribution of Inactivated Tick-Borne Encephalitis Virus Particles Revealed by a Comprehensive Physicochemical Approach" Biomedicines 10, no. 10: 2478. https://doi.org/10.3390/biomedicines10102478
APA StyleMoiseenko, A. V., Bagrov, D. V., Vorovitch, M. F., Uvarova, V. I., Veselov, M. M., Kashchenko, A. V., Ivanova, A. L., Osolodkin, D. I., Egorov, A. M., Ishmukhametov, A. A., Shaitan, K. V., & Sokolova, O. S. (2022). Size Distribution of Inactivated Tick-Borne Encephalitis Virus Particles Revealed by a Comprehensive Physicochemical Approach. Biomedicines, 10(10), 2478. https://doi.org/10.3390/biomedicines10102478