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Article

Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients

1
Department of Biochemistry and Biomedicine, School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK
2
Department of Translational Genomics, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA
3
Department of Neurosurgery, University Hospitals Sussex, Brighton BN2 5BE, UK
4
Royal College of Surgeons in Ireland, D02 YN77 Dublin, Ireland
5
Department of Neurosurgery, Beaumont Hospital, D09 V2N0 Dublin, Ireland
6
Centre for Stress and Age Related Diseases, School of Applied Sciences, University of Brighton, Brighton BN2 4GJ, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Miguel Idoate
Biomedicines 2022, 10(1), 125; https://doi.org/10.3390/biomedicines10010125
Received: 23 November 2021 / Revised: 5 January 2022 / Accepted: 5 January 2022 / Published: 7 January 2022
(This article belongs to the Special Issue Extracellular Vesicles in Cancers)
Glioblastoma (GB) is an aggressive type of tumour for which therapeutic options and biomarkers are limited. GB diagnosis mostly relies on symptomatic presentation of the tumour and, in turn, brain imaging and invasive biopsy that can delay its diagnosis. Description of easily accessible and effective biomarkers present in biofluids would thus prove invaluable in GB diagnosis. Extracellular vesicles (EVs) derived from both GB and stromal cells are essential to intercellular crosstalk in the tumour bulk, and circulating EVs have been described as a potential reservoir of GB biomarkers. Therefore, EV-based liquid biopsies have been suggested as a promising tool for GB diagnosis and follow up. To identify GB specific proteins, sEVs were isolated from plasma samples of GB patients as well as healthy volunteers using differential ultracentrifugation, and their content was characterised through mass spectrometry. Our data indicate the presence of an inflammatory biomarker signature comprising members of the complement and regulators of inflammation and coagulation including VWF, FCGBP, C3, PROS1, and SERPINA1. Overall, this study is a step forward in the development of a non-invasive liquid biopsy approach for the identification of valuable biomarkers that could significantly improve GB diagnosis and, consequently, patients’ prognosis and quality of life. View Full-Text
Keywords: glioblastoma; extracellular vesicles; liquid biopsy glioblastoma; extracellular vesicles; liquid biopsy
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MDPI and ACS Style

Cilibrasi, C.; Simon, T.; Vintu, M.; Tolias, C.; Samuels, M.; Mazarakis, N.K.; Eravci, M.; Stewart, N.; Critchley, G.; Giamas, G. Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients. Biomedicines 2022, 10, 125. https://doi.org/10.3390/biomedicines10010125

AMA Style

Cilibrasi C, Simon T, Vintu M, Tolias C, Samuels M, Mazarakis NK, Eravci M, Stewart N, Critchley G, Giamas G. Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients. Biomedicines. 2022; 10(1):125. https://doi.org/10.3390/biomedicines10010125

Chicago/Turabian Style

Cilibrasi, Chiara, Thomas Simon, Marian Vintu, Christos Tolias, Mark Samuels, Nektarios K. Mazarakis, Murat Eravci, Nicolas Stewart, Giles Critchley, and Georgios Giamas. 2022. "Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients" Biomedicines 10, no. 1: 125. https://doi.org/10.3390/biomedicines10010125

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