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Article

Seed Total Protein Profiling in Discrimination of Closely Related Pines: Evidence from the Pinus mugo Complex

1
Department of Genetics, Institute of Experimental Biology, Faculty of Biology, School of Natural Sciences, Adam Mickiewicz University, Uniwersytetu Poznańskiego 6, 61-614 Poznań, Poland
2
Department of Biochemistry and Food Analysis, Faculty of Food and Nutrition Sciences, University of Life Sciences, Mazowiecka 48, 60-623 Poznań, Poland
3
Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, Tr. A. Hlinku 1, 949 74 Nitra, Slovakia
4
Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland
5
Laboratory of Plant Physiology, Department of Plant Biology and Ecology, Faculty of Biology, University of Bialystok, Ciołkowskiego 1J, 15-245 Białystok, Poland
*
Author to whom correspondence should be addressed.
Plants 2020, 9(7), 872; https://doi.org/10.3390/plants9070872
Received: 12 June 2020 / Revised: 29 June 2020 / Accepted: 7 July 2020 / Published: 9 July 2020
(This article belongs to the Special Issue Systematics and the Conservation of Plant Diversity)
The Pinus mugo complex includes several dozen closely related European mountain pines. The discrimination of specific taxa within this complex is still extremely challenging, although numerous methodologies have been used to solve this problem, including morphological and anatomical analyses, cytological studies, allozyme variability, and DNA barcoding, etc. In this study, we used the seed total protein (STP) patterns to search for taxonomically interesting differences among three closely-related pine taxa from the Pinus mugo complex and five more distant species from the Pinaceae family. It was postulated that STP profiling can serve as the backup methodology for modern taxonomic research, in which more sophisticated analyses, i.e., based on the DNA barcoding approach, have been found to be useless. A quantitative analysis of the STP profiles revealed characteristic electrophoretic patterns for all the analyzed taxa from Pinaceae. STP profiling enabled the discrimination of closely-related pine taxa, even of those previously indistinguishable by chloroplast DNA barcodes. The results obtained in this study indicate that STP profiling can be very useful for solving complex taxonomic puzzles. View Full-Text
Keywords: seed total proteins; taxonomic discrimination; SDS-PAGE protein profiling; species complex; Pinaceae seed total proteins; taxonomic discrimination; SDS-PAGE protein profiling; species complex; Pinaceae
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MDPI and ACS Style

Celiński, K.; Sokołowska, J.; Zemleduch-Barylska, A.; Kuna, R.; Kijak, H.; Staszak, A.M.; Wojnicka-Półtorak, A.; Chudzińska, E. Seed Total Protein Profiling in Discrimination of Closely Related Pines: Evidence from the Pinus mugo Complex. Plants 2020, 9, 872. https://doi.org/10.3390/plants9070872

AMA Style

Celiński K, Sokołowska J, Zemleduch-Barylska A, Kuna R, Kijak H, Staszak AM, Wojnicka-Półtorak A, Chudzińska E. Seed Total Protein Profiling in Discrimination of Closely Related Pines: Evidence from the Pinus mugo Complex. Plants. 2020; 9(7):872. https://doi.org/10.3390/plants9070872

Chicago/Turabian Style

Celiński, Konrad, Joanna Sokołowska, Agata Zemleduch-Barylska, Roman Kuna, Hanna Kijak, Aleksandra M. Staszak, Aleksandra Wojnicka-Półtorak, and Ewa Chudzińska. 2020. "Seed Total Protein Profiling in Discrimination of Closely Related Pines: Evidence from the Pinus mugo Complex" Plants 9, no. 7: 872. https://doi.org/10.3390/plants9070872

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