Next Article in Journal
Selenium Enrichment Enhances the Quality and Shelf Life of Basil Leaves
Previous Article in Journal
The Essential Oil of Salvia rosmarinus Spenn. from Italy as a Source of Health-Promoting Compounds: Chemical Profile and Antioxidant and Cholinesterase Inhibitory Activity
Open AccessArticle

Evolutionary Understanding of Aquaporin Transport System in the Basal Eudicot Model Species Aquilegia coerulea

1
National Agri-Food Biotechnology Institute (NABI), Mohali Punjab 140306, India
2
Department of Botany, Savitribai Phule Pune University, Pune, Maharashtra 411007, India
3
Department of Biotechnology, Panjab University, Chandigarh 160014, India
4
Division of Crop Science, Indian Council of Agricultural Research, Krishi Bhavan, New Delhi 110001, India
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(6), 799; https://doi.org/10.3390/plants9060799
Received: 7 March 2020 / Revised: 7 June 2020 / Accepted: 22 June 2020 / Published: 26 June 2020
Aquaporins (AQPs) play a pivotal role in the cellular transport of water and many other small solutes, influencing many physiological and developmental processes in plants. In the present study, extensive bioinformatics analysis of AQPs was performed in Aquilegia coerulea L., a model species belonging to basal eudicots, with a particular focus on understanding the AQPs role in the developing petal nectar spur. A total of 29 AQPs were identified in Aquilegia, and their phylogenetic analysis performed with previously reported AQPs from rice, poplar and Arabidopsis depicted five distinct subfamilies of AQPs. Interestingly, comparative analysis revealed the loss of an uncharacterized intrinsic protein II (XIP-II) group in Aquilegia. The absence of the entire XIP subfamily has been reported in several previous studies, however, the loss of a single clade within the XIP family has not been characterized. Furthermore, protein structure analysis of AQPs was performed to understand pore diversity, which is helpful for the prediction of solute specificity. Similarly, an AQP AqcNIP2-1 was identified in Aquilegia, predicted as a silicon influx transporter based on the presence of features such as the G-S-G-R aromatic arginine selectivity filter, the spacing between asparagine-proline-alanine (NPA) motifs and pore morphology. RNA-seq analysis showed a high expression of tonoplast intrinsic proteins (TIPs) and plasma membrane intrinsic proteins (PIPs) in the developing petal spur. The results presented here will be helpful in understanding the AQP evolution in Aquilegia and their expression regulation, particularly during floral development. View Full-Text
Keywords: Aquilegia; aquaporins; bioinformatics; NPA motifs; transporter; transcriptomics; floral development Aquilegia; aquaporins; bioinformatics; NPA motifs; transporter; transcriptomics; floral development
Show Figures

Figure 1

MDPI and ACS Style

Singh, S.; Bhatt, V.; Kumar, V.; Kumawat, S.; Khatri, P.; Singla, P.; Shivaraj, S.; Nadaf, A.; Deshmukh, R.; Sharma, T.R.; Sonah, H. Evolutionary Understanding of Aquaporin Transport System in the Basal Eudicot Model Species Aquilegia coerulea. Plants 2020, 9, 799.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop