Secondary Structure of Chloroplast mRNAs In Vivo and In Vitro
Abstract
:1. Introduction
2. Results
2.1. Validation of the Method
2.2. Analysis of the Secondary Structure of Start Codons
2.3. Analysis of the Secondary Structures of Shine-Dalgarno Sequences and Translation Initiation Regions
2.4. Analysis of the Secondary Structures of Coding Regions
2.5. The Secondary Structure of An Antisense tRNA Sequence in the psbD/C/Z Operon
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. RNA Structure Probing with DMS
4.3. cDNA Synthesis
4.4. PCR Amplification
4.5. Data Analysis
4.6. 70S Structure Analysis
4.7. RNA Structure Prediction
4.8. Prediction of Shine-Dalgarno Sequences
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gawroński, P.; Pałac, A.; Scharff, L.B. Secondary Structure of Chloroplast mRNAs In Vivo and In Vitro. Plants 2020, 9, 323. https://doi.org/10.3390/plants9030323
Gawroński P, Pałac A, Scharff LB. Secondary Structure of Chloroplast mRNAs In Vivo and In Vitro. Plants. 2020; 9(3):323. https://doi.org/10.3390/plants9030323
Chicago/Turabian StyleGawroński, Piotr, Aleksandra Pałac, and Lars B. Scharff. 2020. "Secondary Structure of Chloroplast mRNAs In Vivo and In Vitro" Plants 9, no. 3: 323. https://doi.org/10.3390/plants9030323