Molecular Basis of Lipid Metabolism in Oryza sativa L.

Round 1

Reviewer 1 Report

Comments and Suggestions for Authors

Molecular Basis of Lipid Metabolism in Oryza sativa L.

1. The manuscript provides a comprehensive analysis of the rice transcriptome and lipidome, offering valuable insights into lipid metabolism's role in cold tolerance.
2. The integration of multiple research methods strengthens the validity of the findings.
3. The study addresses a significant gap in understanding rice's response to cold stress.
4. The authors have included a broad range of references, indicating thorough research.
5. The discussion section effectively links the results to broader implications for rice agriculture.
6. The visual data representation is clear and aids in understanding complex concepts.
7. The manuscript is well-structured, with a logical flow of information.
8. The language used is precise and accessible to a wide audience.
9. The study's findings could have practical applications in improving rice cultivation under cold conditions.
10. The authors have identified potential areas for further research, demonstrating a forward-thinking approach.

Negative comments:

1. The manuscript could benefit from additional data on long-term cold tolerance effects.
2. Some sections lack detailed explanations of methodologies used.
3. The sample size for certain experiments is relatively small, which may affect the generalizability of the results.
4. There is limited discussion on the potential limitations of the study.
5. The introduction could be expanded to provide more context on previous studies.
6. Some figures are difficult to interpret without further explanation.
7. The manuscript would benefit from a more detailed conclusion summarizing key findings.
8. There is a need for clearer definitions of technical terms for non-specialist readers.
9. Some references are outdated and should be updated with recent studies.
10. The impact of lipid metabolism on other stress factors besides cold is not thoroughly explored.

Comments on the Quality of English Language

Molecular Basis of Lipid Metabolism in Oryza sativa L.

1. The manuscript provides a comprehensive analysis of the rice transcriptome and lipidome, offering valuable insights into lipid metabolism's role in cold tolerance.
2. The integration of multiple research methods strengthens the validity of the findings.
3. The study addresses a significant gap in understanding rice's response to cold stress.
4. The authors have included a broad range of references, indicating thorough research.
5. The discussion section effectively links the results to broader implications for rice agriculture.
6. The visual data representation is clear and aids in understanding complex concepts.
7. The manuscript is well-structured, with a logical flow of information.
8. The language used is precise and accessible to a wide audience.
9. The study's findings could have practical applications in improving rice cultivation under cold conditions.
10. The authors have identified potential areas for further research, demonstrating a forward-thinking approach.

Negative comments:

1. The manuscript could benefit from additional data on long-term cold tolerance effects.
2. Some sections lack detailed explanations of methodologies used.
3. The sample size for certain experiments is relatively small, which may affect the generalizability of the results.
4. There is limited discussion on the potential limitations of the study.
5. The introduction could be expanded to provide more context on previous studies.
6. Some figures are difficult to interpret without further explanation.
7. The manuscript would benefit from a more detailed conclusion summarizing key findings.
8. There is a need for clearer definitions of technical terms for non-specialist readers.
9. Some references are outdated and should be updated with recent studies.
10. The impact of lipid metabolism on other stress factors besides cold is not thoroughly explored.
11. This manuscript requires minor revision before acceptance for publication.

Author Response

Reviewer Comments:
Reviewer 1:
1. The manuscript could benefit from additional data on long-term cold tolerance effects.

Response: Thank you very much for your suggestions. We have supplemented the effect of lipid metabolism on cold tolerance in rice (Lines 116 to 127).

 

2. Some sections lack detailed explanations of methodologies used.

Response: Thank you for your suggestion. We have explained the methodologies used in detail in this article based on previous research.

 

3. The sample size for certain experiments is relatively small, which may affect the generalizability of the results.

Response: In our review article, we have showed the sample size for certain experiments according to the original articles.

 

4. There is limited discussion on the potential limitations of the study.

Response: We have supplemented the potential limitations of lipid metabolism in rice (Lines 436 to 448).

 

5. The introduction could be expanded to provide more context on previous studies.

Response: Thank you for your suggestion. We have expanded on previous studies of lipid metabolism in rice in the introduction and the previous research is also supplemented in 1.2.1 below. (Lines 52 to 56, Lines 116 to 141).

 

6. Some figures are difficult to interpret without further explanation.

Response: We have optimized Figure.1, Figure.2 and Figure.3

 

7. The manuscript would benefit from a more detailed conclusion summarizing key findings.

Response: We have supplemented the conclusions. (Lines 436 to 448).

 

8. There is a need for clearer definitions of technical terms for non-specialist readers.
   Response: We have explained some of the technical terms in the manuscript.

 

9. Some references are outdated and should be updated with recent studies.

Response: Thank you for your suggestion. We have updated the references.

 

10. The impact of lipid metabolism on other stress factors besides cold is not thoroughly explored.

Response: Thank you very much for your review. We have supplemented the effect of stress factors besides cold of lipid metabolism (Lines 128 to 141).

Author Response File: Author Response.pdf

Reviewer 2 Report

Comments and Suggestions for Authors

Dear Authors,

I have reviewed the manuscript and have the following observations:

The topic of the manuscript is the molecular basis of lipid metabolism, which provides an essential foundation for genetic improvement of rice quality. 

The Abstract is adequate but short. I suggest expanding it to include more important topics.

Keywords: I suggest expanding and not using keywords that are not in the title. In addition, what is not a proper noun should not be capitalized

Additional information should not appear as 'note' after the title below the figures. It is recommended to clarify the abbreviations and parts of the figures.

It is also useful to use figures or tables similar to those in point 1 to aid understanding. 

Introduction at the end of the chapter on the purpose of the research, the purpose of the manuscript is not clear. I would therefore recommend including this in the Abstract and in the manuscript.

A Conclusion chapter would be necessary, yet what was the result of the research, what did the authors conclude. 

Author Response

Reviewer 2
I have reviewed the manuscript and have the following observations:

 

The topic of the manuscript is the molecular basis of lipid metabolism, which provides an essential foundation for genetic improvement of rice quality. 

 

1. The Abstract is adequate but short. I suggest expanding it to include more important topics.

Response: Thank you very much for your careful review. We have supplemented the abstract (Lines 19 to 21).

 

2. Keywords: I suggest expanding and not using keywords that are not in the title. In addition, what is not a proper noun should not be capitalized

Response: Thank you for your suggestion. We have corrected the format of the keywords (Line 23).

 

3. Additional information should not appear as 'note' after the title below the figures. It is recommended to clarify the abbreviations and parts of the figures.

Response: Thank you very much for your suggestion. We have corrected the presentation of additional information below the figures (Lines 228 -237, 270-273, 336-337).

 

It is also useful to use figures or tables similar to those in point 1 to aid understanding. 

 

4. Introduction at the end of the chapter on the purpose of the research, the purpose of the manuscript is not clear. I would therefore recommend including this in the Abstract and in the manuscript.

Response: We have further elaborated the purpose of the paper study in the introduction (Lines 52-59).

 

A Conclusion chapter would be necessary, yet what was the result of the research, what did the authors conclude. 

5. Response: Thank you very much for your suggestion. We have supplemented the conclusions. (Lines 413 to 458).

Author Response File: Author Response.pdf

Reviewer 3 Report

Comments and Suggestions for Authors

Dear Authors,

Your manuscript ‘Molecular Basis of Lipid Metabolism in Oryza sativa L.’ is very interesting.

Lipids are presented as the basic biological molecules of plants, with glycerolipids serving as cell membranes and triacylglycerols as a source of energy. In turn, fatty acids are the main components of plant lipids.

In rice, lipids and fatty acids have a significant influence on quality

Through genetic analysis, significant progress has been made in discovering the functional mechanisms and regulatory pathways of lipid metabolism, including the biological synthesis and degradation of fatty acids, glycerolipids and triacylglycerols in rice.

However, the quantitative trait loci (QTL) identified through the analysis of natural variations in the composition and content of lipids and fatty acids were integrated and represented on 12 chromosomes.

These studies highlight the understanding of the molecular basis of lipid metabolism and establish a substantial basis for the genetic improvement of rice quality.

I believe that the manuscript is of potential interest for readers.

My specific comments, that I hope will help the authors to improve the manuscript:

 

Abstract:

In my opinion it is well structured, and the most important conclusions of the study are well presented there.

 

1.Introduction:

Point 1 - I suggest you write a bit about rice so that you have an introduction to rice cultivation. Start by doing so, but then quickly move on to lipids. It's understandable because it's the main topic of the article, but in my opinion there's a lack of 2/3 lines talking about the crop.

1.1- They frame the topic well, presenting concrete values (%) of the elements they mention.

1.2 - They indicate the functions of lipids in the growth and development of rice and then in the next point Roles of Lipids in Rice Quality - nothing to add on these topics! Well done.

 

2. Biosynthesis and Metabolism in Rice Lipid:

Point 2.1 - It is difficult for the reader to understand all the concepts just from what they have written. It is easier to understand the information they want to convey by using the image they have created.

Is the image your own? If it isn't, you need to indicate the source from which you took it or if you adapted it by writing it yourself.

Point 2.2 - The same as in the previous point. You don't show the source of the image. Use up-to-date and relevant information in the text

 

3.Genetic Basis for Controlling Rice Lipid and Fatty Acid Content:

Point 3.1 - There are many acronyms throughout the text, which makes it difficult for the reader to interpret. The image is not very easy to understand the information they are trying to convey. In my opinion, they could use a different graphic look.

 

Point 3.2 - Relevant and current studies on the Molecular Regulation of Rice Lipid Metabolism are presented.

 

4.Summary:

summarize the main points with an up-to-date bibliography (as far as possible)

 

References:

It’s ok.

Hyperlinked (URL) references are shown (underlined and in blue). Other DOIs where this is not the case are also shown. I suggest that it be reformulated and everything made uniform in terms of formatting.

Author Response

Reviewer 3:

Your manuscript ‘Molecular Basis of Lipid Metabolism in Oryza sativa L.’ is very interesting.

Lipids are presented as the basic biological molecules of plants, with glycerolipids serving as cell membranes and triacylglycerols as a source of energy. In turn, fatty acids are the main components of plant lipids.

In rice, lipids and fatty acids have a significant influence on quality

Through genetic analysis, significant progress has been made in discovering the functional mechanisms and regulatory pathways of lipid metabolism, including the biological synthesis and degradation of fatty acids, glycerolipids and triacylglycerols in rice.

However, the quantitative trait loci (QTL) identified through the analysis of natural variations in the composition and content of lipids and fatty acids were integrated and represented on 12 chromosomes.

These studies highlight the understanding of the molecular basis of lipid metabolism and establish a substantial basis for the genetic improvement of rice quality.

I believe that the manuscript is of potential interest for readers.

My specific comments, that I hope will help the authors to improve the manuscript:

 

Abstract:

In my opinion it is well structured, and the most important conclusions of the study are well presented there.

 

1.Introduction:

Point 1 - I suggest you write a bit about rice so that you have an introduction to rice cultivation. Start by doing so, but then quickly move on to lipids. It's understandable because it's the main topic of the article, but in my opinion there's a lack of 2/3 lines talking about the crop.

Response: Thank you very much for your suggestion. We have added to the content on rice cultivation (Lines 28 to 33).

 

1.1- They frame the topic well, presenting concrete values (%) of the elements they mention.

1.2 - They indicate the functions of lipids in the growth and development of rice and then in the next point Roles of Lipids in Rice Quality - nothing to add on these topics! Well done.

 

2. Biosynthesis and Metabolism in Rice Lipid:

Point 2.1 - It is difficult for the reader to understand all the concepts just from what they have written. It is easier to understand the information they want to convey by using the image they have created.

Is the image your own? If it isn't, you need to indicate the source from which you took it or if you adapted it by writing it yourself.

Point 2.2 - The same as in the previous point. You don't show the source of the image. Use up-to-date and relevant information in the text.

 Response Point 2.1 and Point 2.2: Thank you very much for your careful review. The image in the article was actually made by us.

 

3.Genetic Basis for Controlling Rice Lipid and Fatty Acid Content:

Point 3.1 - There are many acronyms throughout the text, which makes it difficult for the reader to interpret. The image is not very easy to understand the information they are trying to convey. In my opinion, they could use a different graphic look.

Response: We have reduced the use of acronyms in the manuscript (Lines 194-222), and optimized the graphical appearance (Fig.1 and Fig.2).

 

Point 3.2 - Relevant and current studies on the Molecular Regulation of Rice Lipid Metabolism are presented.

 

4.Summary:

summarize the main points with an up-to-date bibliography (as far as possible)

Response: We have updated the bibliography of the manuscript.

 

5.References:

It’s ok.

Hyperlinked (URL) references are shown (underlined and in blue). Other DOIs where this is not the case are also shown. I suggest that it be reformulated and everything made uniform in terms of formatting.

Response: Thank you very much for your review. We have corrected the format of the reference.

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

Comments and Suggestions for Authors

I recommand it in this form to publication.