Next Article in Journal
Comparison of Proanthocyanidin Content in Rabbiteye Blueberry (Vaccinium virgatum Aiton) Leaves and the Promotion of Apoptosis against HL-60 Promyelocytic Leukemia Cells Using ‘Kunisato 35 Gou’ Leaf Extract
Previous Article in Journal
Hydrogen Gas Improves the Postharvest Quality of Lanzhou Lily (Lilium davidii var. unicolor) Bulbs
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Plants
Volume 12
Issue 4
10.3390/plants12040947
plants-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential

by
Yoana Kizheva
1,*,
Zoltan Urshev
2,
Melani Dimitrova
1,
Nevena Bogatzevska
3,
Penka Moncheva
1 and
Petya Hristova
1
1
Department of General and Industrial Microbiology, Faculty of Biology, Sofia University St. “Kliment Ohridski”, 8 Dragan Tsankov Blvd., 1000 Sofia, Bulgaria
2
R&D Department, LB Bulgaricum PLC, 14 Malashevska Str., 1000 Sofia, Bulgaria
3
Institute of Soil Science, Agrotechnologies and Plant Protection “N. Poushkarov”, Agricultural Academy, 1331 Sofia, Bulgaria
*
Author to whom correspondence should be addressed.
Plants 2023, 12(4), 947; https://doi.org/10.3390/plants12040947
Submission received: 23 December 2022 / Revised: 10 February 2023 / Accepted: 16 February 2023 / Published: 19 February 2023
(This article belongs to the Section Plant Protection and Biotic Interactions)
Browse Figures
Versions Notes

Abstract

Bacteriophages have greatly engaged the attention of scientists worldwide due to the continuously increasing resistance of phytopathogenic bacteria to commercially used chemical pesticides. However, the knowledge regarding phages is still very insufficient and must be continuously expanded. This paper presents the results of the isolation, characterization, and evaluation of the potential of 11 phage isolates as natural predators of a severe phytopathogenic bacterium—Xanthomonas euvesicatoria. Phages were isolated from the rhizosphere of tomato plants with symptoms of bacterial spot. The plaque morphology of all isolates was determined on a X. euvesicatoria lawn via a plaque assay. Three of the isolates were attributed to the family Myoviridae based on TEM micrographs. All phages showed good long-term viability when stored at 4 °C and −20 °C. Three of the phage isolates possessed high stability at very low pH values. Fifty-five-day persistence in a soil sample without the presence of the specific host and a lack of lytic activity on beneficial rhizosphere bacteria were found for the phage isolate BsXeu269p/3. The complete genome of the same isolate was sequenced and analyzed, and, for the first time in this paper, we report a circular representation of a linear but circularly permuted phage genome among known X. euvesicatoria phage genomes.
Keywords: Myoviridae bacteriophages; bacterial spot disease; Xanthomonas euvesicatoria; circular genome permutation; rhizosphere Myoviridae bacteriophages; bacterial spot disease; Xanthomonas euvesicatoria; circular genome permutation; rhizosphere

Share and Cite

MDPI and ACS Style

Kizheva, Y.; Urshev, Z.; Dimitrova, M.; Bogatzevska, N.; Moncheva, P.; Hristova, P. Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential. Plants 2023, 12, 947. https://doi.org/10.3390/plants12040947

AMA Style

Kizheva Y, Urshev Z, Dimitrova M, Bogatzevska N, Moncheva P, Hristova P. Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential. Plants. 2023; 12(4):947. https://doi.org/10.3390/plants12040947

Chicago/Turabian Style

Kizheva, Yoana, Zoltan Urshev, Melani Dimitrova, Nevena Bogatzevska, Penka Moncheva, and Petya Hristova. 2023. "Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential" Plants 12, no. 4: 947. https://doi.org/10.3390/plants12040947

APA Style

Kizheva, Y., Urshev, Z., Dimitrova, M., Bogatzevska, N., Moncheva, P., & Hristova, P. (2023). Phenotypic and Genotypic Characterization of Newly Isolated Xanthomonas euvesicatoria-Specific Bacteriophages and Evaluation of Their Biocontrol Potential. Plants, 12(4), 947. https://doi.org/10.3390/plants12040947

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop