Optimization of Heterotrophic Culture Conditions for the Algae Graesiella emersonii WBG-1 to Produce Proteins
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effects of Carbon Source and Initial Concentration on Growth and Protein Yield of G. emersonii WBG-1
2.2. Effects of Nitrogen Sources and Initial Nitrogen Concentration on the Growth, Protein Content, and Yield of G. emersonii WBG-1
2.3. Effect of Temperature on Heterotrophic Growth, Protein Content, and Yield of G. emersonii WBG-1
3. Materials and Methods
3.1. Microalgal Strain
3.2. Algae Seed Culture Conditions
3.3. Optimization of the Carbon Source
3.4. Optimization of Nitrogen Source
3.5. Optimization of Fermentation Temperature
3.6. Analytical Procedures
3.7. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Carbon Source | Initial Concentration (g·L−1) | Biomass (g·L−1) | Protein Content (%, DW) | Protein Yield (g·L−1) |
---|---|---|---|---|
Glucose | 30 | 15.36 ± 0.23 | 34.19 ± 0.53 | 5.25 ± 0.12 |
Sodium acetate | 30 | 4.45 ± 0.15 | 38.89 ± 0.42 | 1.73 ± 0.06 |
Sucrose | 30 | — | — | — |
Species | Cultivation Mode | Protein Content (% DW) | Reference |
---|---|---|---|
Graesiella emersonii WBG-1 | heterotrophic | 66.1 | This study |
Chlorella sp. CMBB276 | heterotrophic | 37.3 | [38] |
Chlorella vulgaris | heterotrophic | 48.7 | [43] |
Chlorella sorokiniana | heterotrophic | 16.3 | [16] |
Chlorella vulgaris | heterotrophic | 36.5 | [44] |
Chlorella vulgaris | two-stage heterotrophic | 44.3 | [21] |
Chlorella vulgaris | two-stage heterotrophic | 59.8 | [37] |
Chlorella | sequential heterotrophic/autotrophic | 60.1 | [18] |
Chlorella vulgaris | Heterotrophy–dilution–photoinduction | 50.9 | [20] |
Chlorella vulgaris | mixotrophic | 58.4 | [19] |
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Wang, K.; Wang, Z.; Ding, Y.; Yu, Y.; Wang, Y.; Geng, Y.; Li, Y.; Wen, X. Optimization of Heterotrophic Culture Conditions for the Algae Graesiella emersonii WBG-1 to Produce Proteins. Plants 2023, 12, 2255. https://doi.org/10.3390/plants12122255
Wang K, Wang Z, Ding Y, Yu Y, Wang Y, Geng Y, Li Y, Wen X. Optimization of Heterotrophic Culture Conditions for the Algae Graesiella emersonii WBG-1 to Produce Proteins. Plants. 2023; 12(12):2255. https://doi.org/10.3390/plants12122255
Chicago/Turabian StyleWang, Kaixuan, Zhongjie Wang, Yi Ding, Youzhi Yu, Yali Wang, Yahong Geng, Yeguang Li, and Xiaobin Wen. 2023. "Optimization of Heterotrophic Culture Conditions for the Algae Graesiella emersonii WBG-1 to Produce Proteins" Plants 12, no. 12: 2255. https://doi.org/10.3390/plants12122255