In Dormant Red Rice Seeds, the Inhibition of Early Seedling Growth, but Not of Germination, Requires Extracellular ABA
Abstract
:1. Introduction
2. Results and Discussion
2.1. Xanthoxal Characterization
2.2. Germination Test
3. Materials and Methods
3.1. Chemicals
3.2. Xanthoxal Production
3.2.1. Extraction of Xanthophylls
3.2.2. Liquid-Liquid Partition of Xanthophylls
3.2.3. Purification of Violaxanthin and Neoxanthin with Solid-Phase Extraction
3.2.4. Production of Xanthoxal by Oxidation of Epoxydic Xanthophylls
3.2.5. Purification of Xanthoxal with Reversed-Phase SPE
3.2.6. Photoisomerization
3.2.7. Final Purification of Xanthoxal with Normal-Phase SPE
3.3. Chemical Analyses
3.3.1. HPLC Analyses
3.3.2. Mass Spectrometry
3.4. Chemical Editor
3.5. Seed Materials and Experimental Setup
3.6. Statistical Analysis
4. Conclusions
Supplementary Materials
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Statistical Details and Notes
/* conditional model with continuous time */ proc GLIMMIX method = Laplace; effect spltime = spline(day); class rep exp trt; model VAR/n = trt trt * spltime/link = probit; random trt * exp; random rep(trt * exp)/type = AR(1); lsmeans trt/at day = 10 adjust = sidak stepdown ilink; lsmeans trt/at day = 20 adjust = sidak stepdown ilink; lsmestimate trt ‘D vs. D + F + A’ 1 0 −1 0 0, ‘D vs. D + F + X’ 1 0 0 −1 0, ‘D + F + A vs. D + F + X’ 0 0 1 −1 0/ at day = 20 adjust = sidak stepdown elsm; run; |
Type III Tests of Fixed Effects | ||||
---|---|---|---|---|
Effect | Num DF | Den DF | F Value | Pr > F |
trt | 4 | 726 | 0.72 | 0.5802 |
spltime*trt | 26 | 726 | 49.47 | <0.0001 |
Type III Tests of Fixed Effects | ||||
---|---|---|---|---|
Effect | Num DF | Den DF | F Value | Pr > F |
trt | 4 | 726 | 0.37 | 0.8305 |
spltime*trt | 26 | 726 | 27.63 | <0.0001 |
Type III Tests of Fixed Effects | ||||
---|---|---|---|---|
Effect | Num DF | Den DF | F Value | Pr > F |
trt | 4 | 726 | 0.11 | 0.9796 |
spltime*trt | 23 | 726 | 18.61 | <0.0001 |
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Gianinetti, A. In Dormant Red Rice Seeds, the Inhibition of Early Seedling Growth, but Not of Germination, Requires Extracellular ABA. Plants 2022, 11, 1023. https://doi.org/10.3390/plants11081023
Gianinetti A. In Dormant Red Rice Seeds, the Inhibition of Early Seedling Growth, but Not of Germination, Requires Extracellular ABA. Plants. 2022; 11(8):1023. https://doi.org/10.3390/plants11081023
Chicago/Turabian StyleGianinetti, Alberto. 2022. "In Dormant Red Rice Seeds, the Inhibition of Early Seedling Growth, but Not of Germination, Requires Extracellular ABA" Plants 11, no. 8: 1023. https://doi.org/10.3390/plants11081023