Characterization of the TCP Gene Family in Chrysanthemum nankingense and the Role of CnTCP4 in Cold Tolerance
Abstract
:1. Introduction
2. Results
2.1. Classification and Phylogenetic Analysis of TCP Proteins
2.2. Structural Analysis and Protein–Protein Interactions of CnTCP Genes
2.3. Expression Profiling of CnTCP Genes in Response to Cold Stress
2.4. CnTCP4 Transcription Factors Are Involved in Cold Stress Response
3. Discussion
4. Materials and Methods
4.1. Phylogenetic Analysis of the CnTCP Family
4.2. Structural Analysis of the TCP Genes
4.3. Protein–Protein Interaction Network Analysis
4.4. Putative Cis-Elements in the Promoter Regions
4.5. Plant Growth Conditions and Cold Treatment
4.6. Verification of CnTCP Expression
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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CnTCPS | CnTCP4-1 | CnTCP4-3 | CnTCP4-2 | CnTCP14 | CnTCP2-3 | CnTCP2-2 | CnTCP2-1 | CnTCP13 | CnTCP5 | CnTCP17 | CnTCP3 | CnTCP15 |
---|---|---|---|---|---|---|---|---|---|---|---|---|
CnTCP10 | 10.70% | 11.40% | 11.00% | 11.60% | 11.80% | 12.10% | 12.10% | 10.30% | 12.90% | 16.30% | 12.30% | 11.20% |
CnTCP12 | 11.20% | 11.90% | 11.20% | 12.40% | 11.30% | 11.40% | 11.30% | 10.60% | 14.20% | 15.50% | 12.00% | 11.20% |
CnTCP16 | 14.10% | 14.20% | 14.10% | 13.10% | 15.50% | 15.50% | 15.50% | 12.90% | 13.80% | 16.10% | 15.50% | 15.20% |
CnTCP9 | 15.80% | 15.70% | 15.70% | 11.80% | 12.60% | 12.70% | 12.70% | 13.20% | 18.80% | 17.60% | 15.10% | 14.40% |
CnTCP7 | 15.30% | 14.80% | 14.80% | 14.70% | 14.80% | 15.00% | 14.80% | 12.00% | 14.20% | 13.80% | 15.50% | 16.80% |
CnTCP8 | 14.00% | 13.40% | 13.70% | 9.40% | 11.00% | 11.10% | 11.00% | 12.20% | 12.90% | 12.80% | 17.40% | 14.10% |
CnTCP18-1 | 11.80% | 11.90% | 11.80% | 13.40% | 13.60% | 13.60% | 13.80% | 14.20% | 15.50% | 18.10% | 16.40% | 16.10% |
CnTCP18-2 | 12.30% | 12.40% | 12.30% | 14.00% | 13.70% | 13.70% | 14.00% | 14.70% | 16.00% | 17.80% | 16.50% | 16.20% |
CnTCP6 | 16.40% | 16.10% | 16.40% | 15.70% | 14.90% | 14.70% | 14.60% | 15.80% | 17.60% | 17.40% | 14.80% | 15.80% |
CnTCP1 | 19.80% | 20.60% | 20.60% | 19.10% | 17.60% | 17.60% | 17.50% | 17.50% | 19.70% | 22.50% | 31.10% | 29.20% |
CnTCP11 | 20.70% | 21.50% | 20.80% | 15.70% | 17.40% | 17.40% | 17.30% | 17.30% | 14.90% | 19.10% | 27.00% | 25.90% |
CnTCP15 | 16.00% | 16.50% | 16.00% | 17.60% | 18.80% | 18.80% | 18.70% | 16.00% | 16.20% | 19.90% | 46.20% | 100% |
CnTCP3 | 16.50% | 15.70% | 16.10% | 15.40% | 17.00% | 17.00% | 16.90% | 16.70% | 17.80% | 17.90% | 100% | |
CnTCP17 | 20.10% | 20.30% | 20.40% | 25.30% | 26.20% | 26.10% | 26.00% | 37.20% | 52.10% | 100% | ||
CnTCP5 | 19.50% | 20.10% | 19.50% | 23.90% | 23.70% | 23.70% | 23.60% | 34.70% | 100% | |||
CnTCP13 | 19.90% | 21.00% | 21.00% | 26.60% | 24.60% | 24.60% | 24.80% | 100% | ||||
CnTCP2-1 | 20.20% | 19.40% | 20.00% | 57.40% | 99.50% | 99.80% | 100% | |||||
CnTCP2-2 | 20.30% | 19.50% | 20.10% | 57.10% | 99.80% | 100% | ||||||
CnTCP2-3 | 20.40% | 19.60% | 20.10% | 57.10% | 100% | |||||||
CnTCP14 | 22.50% | 21.90% | 22.40% | 100% | ||||||||
CnTCP4-2 | 93.80% | 96.00% | 100% | |||||||||
CnTCP4-3 | 90.10% | 100% | ||||||||||
CnTCP4-1 | 100% | |||||||||||
CnTCPS | CnTCP11 | CnTCP1 | CnTCP6 | CnTCP18-2 | CnTCP18-1 | CnTCP8 | CnTCP7 | CnTCP9 | CnTCP16 | CnTCP12 | CnTCP10 | |
CnTCP10 | 12.40% | 11.90% | 26.80% | 28.00% | 28.30% | 34.20% | 28.70% | 36.00% | 50.00% | 68.10% | 100% | |
CnTCP12 | 11.60% | 13.30% | 26.40% | 28.40% | 28.60% | 34.00% | 29.10% | 34.00% | 50.60% | 100% | ||
CnTCP16 | 11.50% | 13.80% | 27.40% | 31.30% | 31.70% | 38.10% | 35.70% | 39.30% | 100% | |||
CnTCP9 | 14.80% | 18.20% | 28.70% | 31.50% | 31.50% | 28.90% | 33.10% | 100% | ||||
CnTCP7 | 16.20% | 16.20% | 28.80% | 30.20% | 29.20% | 38.50% | 100% | |||||
CnTCP8 | 13.00% | 14.50% | 28.80% | 29.70% | 29.20% | 100% | ||||||
CnTCP18-1 | 14.10% | 16.20% | 41.70% | 97.30% | 100% | |||||||
CnTCP18-2 | 14.20% | 16.20% | 41.30% | 100% | ||||||||
CnTCP6 | 16.30% | 13.90% | 100% | |||||||||
CnTCP1 | 52.30% | 100% | ||||||||||
CnTCP11 | 100% |
Family | AtTCP | CnTCP | Family | AtTCP | CnTCP |
---|---|---|---|---|---|
AP2 | 24 | 18 | TCP | 22 | 17 |
B3 | 24 | 18 | ARF | 24 | 16 |
bHLH | 24 | 18 | BBR-BPC | 24 | 16 |
bZIP | 24 | 18 | WRKY | 24 | 16 |
C2H2 | 24 | 18 | ZF-HD | 22 | 16 |
Dof | 24 | 18 | LBD | 21 | 16 |
ERF | 24 | 18 | SBP | 21 | 16 |
G2-like | 24 | 18 | C3H | 20 | 16 |
GATA | 24 | 18 | WOX | 22 | 15 |
HD-ZIP | 24 | 18 | CPP | 21 | 15 |
MIKC_MADS | 24 | 18 | EIL | 18 | 15 |
MYB | 24 | 18 | NF-YB | 16 | 15 |
MYB_related | 24 | 18 | E2F/DP | 23 | 14 |
NAC | 24 | 18 | Nin-like | 22 | 14 |
Trihelix | 23 | 18 | RAV | 22 | 14 |
GRAS | 23 | 17 | SRS | 21 | 13 |
ARR-B | 20 | 7 |
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Tian, C.; Zhai, L.; Zhu, W.; Qi, X.; Yu, Z.; Wang, H.; Chen, F.; Wang, L.; Chen, S. Characterization of the TCP Gene Family in Chrysanthemum nankingense and the Role of CnTCP4 in Cold Tolerance. Plants 2022, 11, 936. https://doi.org/10.3390/plants11070936
Tian C, Zhai L, Zhu W, Qi X, Yu Z, Wang H, Chen F, Wang L, Chen S. Characterization of the TCP Gene Family in Chrysanthemum nankingense and the Role of CnTCP4 in Cold Tolerance. Plants. 2022; 11(7):936. https://doi.org/10.3390/plants11070936
Chicago/Turabian StyleTian, Chang, Lisheng Zhai, Wenjing Zhu, Xiangyu Qi, Zhongyu Yu, Haibin Wang, Fadi Chen, Likai Wang, and Sumei Chen. 2022. "Characterization of the TCP Gene Family in Chrysanthemum nankingense and the Role of CnTCP4 in Cold Tolerance" Plants 11, no. 7: 936. https://doi.org/10.3390/plants11070936