Morphological, Molecular, and Biochemical Characterization of a Unique Lentil (Lens culinaris Medik.) Genotype Showing Seed-Coat Color Anomalies Due to Altered Anthocyanin Pathway
Abstract
:1. Introduction
2. Results
2.1. Seed-Coat Color Anomalies
2.2. Molecular Marker Analysis
2.3. Biochemical Studies
2.4. Correlation Analysis
2.5. Cluster and PCA Analysis of Functional Features of Lentil Genotypes
3. Discussion
3.1. Morphological Characterization
3.2. Molecular Characterization
3.3. Biochemical Characterization
3.4. Correlation, Cluster and PCA Analysis
3.5. Probable Effect on the Anthocyanin Biosynthesis Pathway
4. Materials and Methods
4.1. Sowing and Generation-Based Seed-Coat Color Analysis
4.2. SSR Marker Analysis
4.3. Biochemical Studies
4.3.1. Total Phenolic Content (TPC)
4.3.2. Total Flavonoids Content (TFC)
4.3.3. DPPH (1,1-Diphenyl-2-picrylhydrazyl) Scavenging Activity
4.3.4. FRAP (Ferric Reducing Antioxidant Power) Assay
4.3.5. Peroxide Quantification
4.3.6. Total Carotenoid Content (TCC)
4.3.7. Total Ascorbic Acid (TAA)
4.3.8. Total (Crude) Soluble Protein
4.3.9. Total Anthocyanin Content (TAC)
4.4. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Year | Seed-Coat Color-Based Plant Category | Total | ||
---|---|---|---|---|
Brown Seed-Coat Colored Plants | Black Seed-Coat Colored Plants | Mixed Seed-Coat Colored Plants | ||
2018–2019 | 36 | 01 | 75 | 112 |
2019–2020 | 75 | 03 | 164 | 242 |
2020–2021 | 65 | 02 | 102 | 169 |
2018–2019 (Brown Seed Coat) | 2019–2020 | 2018–2019 (Black Seed Coat) | 2019–2020 | 2018–2019 (Spotted Seed Coat) | 2019–2020 | |||
---|---|---|---|---|---|---|---|---|
Progenies | Seed-Coat Color | Progenies | Seed-Coat Color | Progenies | Seed-Coat Color | |||
Plant-1 | SP-1 | Brown | Plant-1 | SP-1 | Mixed | Plant-1 | SP-1 | Brown |
SP-2 | Brown | SP-2 | Brown | SP-2 | Mixed | |||
SP-3 | Mixed | SP-3 | Mixed | SP-3 | Brown | |||
Plant-2 | SP-1 | Black | Plant-2 | SP-1 | Brown | Plant-2 | SP-1 | Mixed |
SP-2 | Mixed | SP-2 | Mixed | SP-2 | Mixed | |||
SP-3 | Mixed | SP-3 | Brown | SP-3 | Mixed | |||
Plant-3 | SP-1 | Mixed | Plant-3 | SP-1 | Mixed | Plant-3 | SP-1 | Mixed |
SP-2 | Mixed | SP-2 | Mixed | SP-2 | Mixed | |||
SP-3 | Mixed | SP-3 | Mixed | SP-3 | Mixed | |||
Plant-4 | SP-1 | Mixed | Plant-4 | SP-1 | Mixed | Plant-4 | SP-1 | Brown |
SP-2 | Mixed | SP-2 | Mixed | SP-2 | Mixed | |||
SP-3 | Mixed | SP-3 | Mixed | SP-3 | Mixed |
S. No. | Samples | TPC (mg GAE/100 g DW) | TFC (mg/g DW) | DPPH scavenging (%) | FRAP (µmol Fe2+/g DW) | H2O2 (nmol/g DW) | TCC (mg/100 g DW) | Crude protein (g/100 g DW) | TAC (mg/g DW) | TAA (mg/100 g DW) |
---|---|---|---|---|---|---|---|---|---|---|
1 | L4717 seeds | 1108.15 ± 32.12 h | 1.91 ± 0.07 d | 35.97 ± 4.67 d | 8.56 ± 0.66 e | 1.64 ± 0.05 c | 2.55 ± 0.09 e | 25.60 ± 0.05 ab | 0.016 ± 0.009 b | 27.94 ± 1.70 fg |
2 | Brown seed- coat seeds | 1206.24 ± 20.53 g | 1.97 ± 0.02 cd | 42.86 ± 3.63 cd | 9.23 ± 0.64 e | 1.76 ± 0.08 c | 2.57 ± 0.10 e | 25.04 ± 0.36 b | 0.018 ± 0.003 b | 29.41 ± 4.80 defg |
3 | Black seed- coat seeds | 1132.99 ± 44.25 h | 2.04 ± 0.17 cd | 36.48 ± 3.67 d | 7.35 ± 0.20 f | 1.78 ± 0.14 c | 2.61 ± 0.11 e | 24.88 ± 0.29 b | 0.042 ± 0.018 a | 28.68 ± 5.02 efg |
4 | Spotted seed- coat seeds | 1047.64 ± 32.29 i | 1.98 ± 0.29 cd | 44.90 ± 5.46 c | 8.82 ± 0.60 e | 1.79 ± 005 c | 2.57 ± 0.14 e | 26.43 ± 1.13 a | 0.033 ± 0.004 ab | 27.21 ± 2.82 g |
5 | L4717 sprouts | 1489.68 ± 11.86 f | 2.41 ± 0.12 bc | 59.45 ± 2.31 ab | 14.37 ± 0.09 d | 2.84 ± 0.14 a | 41.95 ± 0.30 bc | 21.75 ± 0.11 d | ND | 35.29 ± 0.00 cdef |
6 | Brown seed- coat sprouts | 1471.21 ± 12.72 f | 2.62 ± 0.13 b | 46.94 ± 3.23 c | 14.99 ± 0.10 c | 2.95 ± 0.23 a | 42.81 ± 0.21 b | 21.16 ± 0.45 d | ND | 38.24 ± 2.40 abc |
7 | Black seed- coat sprouts | 1639.36 ± 17.94 e | 2.53 ± 0.27 b | 41.92 ± 2.36 cd | 14.75 ± 0.17 d | 2.93 ± 0.02 a | 45.35 ± 1.44 a | 20.80 ± 0.31 d | ND | 36.03 ± 1.47 bcde |
8 | Spotted seed- coat sprouts | 1628.54 ± 18.95 e | 2.39 ± 0.32 bc | 47.10 ± 3.12 c | 14.75 ± 0.40 d | 3.04 ± 0.16 a | 42.72 ± 0.29 b | 23.62 ± 0.54 c | ND | 36.76 ± 2.94 a bcd |
9 | L4717 seedlings | 2107.52 ± 21.72 d | 3.25 ± 0.26 a | 66.46 ± 1.00 a | 46.04 ± 0.34 a | 2.74 ± 0.17 a | 37.95 ± 0.30 bd | 9.82 ± 0.16 f | ND | 42.65 ± 3.80 abc |
10 | Brown seed- coat seedlings | 2332.36 ± 25.76 b | 3.09 ± 0.25 a | 56.55 ± 2.83 b | 44.70 ± 1.59 b | 2.81 ± 0.12 a | 38.81 ± 0.21 d | 9.52 ± 013 f | ND | 44.12 ± 3.40 a |
11 | Black seed- coat seedlings | 2434.27 ± 14.62 a | 3.15 ± 0.17 a | 61.89 ± 3.68 ab | 43.24 ± 0.68 c | 2.41 ± 0.07 b | 41.35 ± 1.44 c | 10.80 ± 0.15 e | ND | 43.97 ± 2.42 a |
12 | Spotted seed- coat seedlings | 2199.24 ± 16.25 c | 3.28 ± 0.07 a | 65.85 ± 1.22 a | 42.85 ± 0.36 c | 2.30 ± 0.07 b | 38.72 ± 0.29 d | 9.93 ± 0.57 ef | ND | 43.38 ± 4.52 ab |
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Mishra, G.P.; Ankita; Aski, M.S.; Tontang, M.T.; Choudhary, P.; Tripathi, K.; Singh, A.; Kumar, R.R.; Thimmegowda, V.; Stobdan, T.; et al. Morphological, Molecular, and Biochemical Characterization of a Unique Lentil (Lens culinaris Medik.) Genotype Showing Seed-Coat Color Anomalies Due to Altered Anthocyanin Pathway. Plants 2022, 11, 1815. https://doi.org/10.3390/plants11141815
Mishra GP, Ankita, Aski MS, Tontang MT, Choudhary P, Tripathi K, Singh A, Kumar RR, Thimmegowda V, Stobdan T, et al. Morphological, Molecular, and Biochemical Characterization of a Unique Lentil (Lens culinaris Medik.) Genotype Showing Seed-Coat Color Anomalies Due to Altered Anthocyanin Pathway. Plants. 2022; 11(14):1815. https://doi.org/10.3390/plants11141815
Chicago/Turabian StyleMishra, Gyan P., Ankita, Muraleedhar S. Aski, Mechiya Tomuilim Tontang, Priti Choudhary, Kuldeep Tripathi, Ajeet Singh, Ranjeet Ranjan Kumar, Vinutha Thimmegowda, Tsering Stobdan, and et al. 2022. "Morphological, Molecular, and Biochemical Characterization of a Unique Lentil (Lens culinaris Medik.) Genotype Showing Seed-Coat Color Anomalies Due to Altered Anthocyanin Pathway" Plants 11, no. 14: 1815. https://doi.org/10.3390/plants11141815