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Article

Transgene CpNAC68 from Wintersweet (Chimonanthus praecox) Improves Arabidopsis Survival of Multiple Abiotic Stresses

1
Chongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
2
Department of Biology, Faculty of Science, York University, Toronto, ON M3J 1P3, Canada
*
Authors to whom correspondence should be addressed.
Academic Editor: Juan Barceló
Plants 2021, 10(7), 1403; https://doi.org/10.3390/plants10071403
Received: 7 June 2021 / Revised: 5 July 2021 / Accepted: 5 July 2021 / Published: 9 July 2021
The NAC (NAM, ATAFs, CUC) family of transcription factors (TFs) play a pivotal role in regulating all processes of the growth and development of plants, as well as responses to biotic and abiotic stresses. Yet, the functions of NACs from non-model plant species remains largely uncharacterized. Here, we characterized the stress-responsive effects of a NAC gene isolated from wintersweet, an ornamental woody plant that blooms in winter when temperatures are low. CpNAC68 is clustered in the NAM subfamily. Subcellular localization and transcriptional activity assays demonstrated a nuclear protein that has transcription activator activities. qRT-PCR analyses revealed that CpNAC68 was ubiquitously expressed in old flowers and leaves. Additionally, the expression of CpNAC68 is induced by disparate abiotic stresses and hormone treatments, including drought, heat, cold, salinity, GA, JA, and SA. Ectopic overexpression of CpNAC68 in Arabidopsis thaliana enhanced the tolerance of transgenic plants to cold, heat, salinity, and osmotic stress, yet had no effect on growth and development. The survival rate and chlorophyll amounts following stress treatments were significantly higher than wild type Arabidopsis, and were accompanied by lower electrolyte leakage and malondialdehyde (MDA) amounts. In conclusion, our study demonstrates that CpNAC68 can be used as a tool to enhance plant tolerance to multiple stresses, suggesting a role in abiotic stress tolerance in wintersweet. View Full-Text
Keywords: CpNAC68; transcription factor; Chimonanthus praecox; abiotic stress; cold tolerance CpNAC68; transcription factor; Chimonanthus praecox; abiotic stress; cold tolerance
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MDPI and ACS Style

Lin, J.; Liu, D.; Wang, X.; Ahmed, S.; Li, M.; Kovinich, N.; Sui, S. Transgene CpNAC68 from Wintersweet (Chimonanthus praecox) Improves Arabidopsis Survival of Multiple Abiotic Stresses. Plants 2021, 10, 1403. https://doi.org/10.3390/plants10071403

AMA Style

Lin J, Liu D, Wang X, Ahmed S, Li M, Kovinich N, Sui S. Transgene CpNAC68 from Wintersweet (Chimonanthus praecox) Improves Arabidopsis Survival of Multiple Abiotic Stresses. Plants. 2021; 10(7):1403. https://doi.org/10.3390/plants10071403

Chicago/Turabian Style

Lin, Jie, Daofeng Liu, Xia Wang, Sajjad Ahmed, Mingyang Li, Nik Kovinich, and Shunzhao Sui. 2021. "Transgene CpNAC68 from Wintersweet (Chimonanthus praecox) Improves Arabidopsis Survival of Multiple Abiotic Stresses" Plants 10, no. 7: 1403. https://doi.org/10.3390/plants10071403

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