Preliminary Identification of Key Genes Controlling Peach Pollen Fertility Using Genome-Wide Association Study
1
Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Science, Zhengzhou 450009, Henan, China
2
Beijing Agro-biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editors: Gianni Barcaccia and Alessandro Vannozzi
Plants 2021, 10(2), 242; https://doi.org/10.3390/plants10020242
Received: 6 January 2021
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Revised: 25 January 2021
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Accepted: 25 January 2021
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Published: 27 January 2021
(This article belongs to the Special Issue Genomics for Plant Breeding 2020–2021)
Previous genetic mapping helped detect a ~7.52 Mb putative genomic region for the pollen fertility trait on peach Chromosome 06 (Chr.06), which was too long for candidate gene characterization. In this study, using the whole-genome re-sequencing data of 201 peach accessions, we performed a genome-wide association study to identify key genes related to peach pollen fertility trait. The significant association peak was detected at Chr.06: 2,116,368 bp, which was in accordance with the previous genetic mapping results, but displayed largely improved precision, allowing for the identification of nine candidate genes. Among these candidates, gene PpABCG26, encoding an ATP-binding cassette G (ABCG) transporter and harboring the most significantly associated SNP (Single Nucleotide Polymorphism) marker in its coding region, was hypothesized to control peach pollen fertility/sterility based on the results of gene function comparison, gene relative expression, and nucleotide sequence analysis. The obtained results will help us to understand the genetic basis of peach pollen fertility trait, and to discover applicable markers for pre-selection in peach.
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MDPI and ACS Style
Huang, Z.; Shen, F.; Chen, Y.; Cao, K.; Wang, L. Preliminary Identification of Key Genes Controlling Peach Pollen Fertility Using Genome-Wide Association Study. Plants 2021, 10, 242. https://doi.org/10.3390/plants10020242
AMA Style
Huang Z, Shen F, Chen Y, Cao K, Wang L. Preliminary Identification of Key Genes Controlling Peach Pollen Fertility Using Genome-Wide Association Study. Plants. 2021; 10(2):242. https://doi.org/10.3390/plants10020242
Chicago/Turabian StyleHuang, Zhenyu, Fei Shen, Yuling Chen, Ke Cao, and Lirong Wang. 2021. "Preliminary Identification of Key Genes Controlling Peach Pollen Fertility Using Genome-Wide Association Study" Plants 10, no. 2: 242. https://doi.org/10.3390/plants10020242
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