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Article

The Collagens DPY-17 and SQT-3 Direct Anterior–Posterior Migration of the Q Neuroblasts in C. elegans

Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66046, USA
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Author to whom correspondence should be addressed.
J. Dev. Biol. 2021, 9(1), 7; https://doi.org/10.3390/jdb9010007
Received: 31 January 2021 / Revised: 15 February 2021 / Accepted: 17 February 2021 / Published: 19 February 2021
(This article belongs to the Special Issue Cell Adhesion Molecules in Development)
Cell adhesion molecules and their extracellular ligands control morphogenetic events such as directed cell migration. The migration of neuroblasts and neural crest cells establishes the structure of the central and peripheral nervous systems. In C. elegans, the bilateral Q neuroblasts and their descendants undergo long-range migrations with left/right asymmetry. QR and its descendants on the right migrate anteriorly, and QL and its descendants on the left migrate posteriorly, despite identical patterns of cell division, cell death, and neuronal generation. The initial direction of protrusion of the Q cells relies on the left/right asymmetric functions of the transmembrane receptors UNC-40/DCC and PTP-3/LAR in the Q cells. Here, we show that Q cell left/right asymmetry of migration is independent of the GPA-16/Gα pathway which regulates other left/right asymmetries, including nervous system L/R asymmetry. No extracellular cue has been identified that guides initial Q anterior versus posterior migrations. We show that collagens DPY-17 and SQT-3 control initial Q direction of protrusion. Genetic interactions with UNC-40/DCC and PTP-3/LAR suggest that DPY-17 and SQT-3 drive posterior migration and might act with both receptors or in a parallel pathway. Analysis of mutants in other collagens and extracellular matrix components indicated that general perturbation of collagens and the extracellular matrix (ECM) did not result in directional defects, and that the effect of DPY-17 and SQT-3 on Q direction is specific. DPY-17 and SQT-3 are components of the cuticle, but a role in the basement membrane cannot be excluded. Possibly, DPY-17 and SQT-3 are part of a pattern in the cuticle and/or basement membrane that is oriented to the anterior–posterior axis of the animal and that is deciphered by the Q cells in a left–right asymmetric fashion. Alternatively, DPY-17 and SQT-3 might be involved in the production or stabilization of a guidance cue that directs Q migrations. In any case, these results describe a novel role for the DPY-17 and SQT-3 collagens in directing posterior Q neuroblast migration. View Full-Text
Keywords: directed neuronal migration; collagen; UNC-40/DCC; PTP-3/LAR; extracellular matrix; C. elegans directed neuronal migration; collagen; UNC-40/DCC; PTP-3/LAR; extracellular matrix; C. elegans
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MDPI and ACS Style

Lang, A.E.; Lundquist, E.A. The Collagens DPY-17 and SQT-3 Direct Anterior–Posterior Migration of the Q Neuroblasts in C. elegans. J. Dev. Biol. 2021, 9, 7. https://doi.org/10.3390/jdb9010007

AMA Style

Lang AE, Lundquist EA. The Collagens DPY-17 and SQT-3 Direct Anterior–Posterior Migration of the Q Neuroblasts in C. elegans. Journal of Developmental Biology. 2021; 9(1):7. https://doi.org/10.3390/jdb9010007

Chicago/Turabian Style

Lang, Angelica E., and Erik A. Lundquist 2021. "The Collagens DPY-17 and SQT-3 Direct Anterior–Posterior Migration of the Q Neuroblasts in C. elegans" Journal of Developmental Biology 9, no. 1: 7. https://doi.org/10.3390/jdb9010007

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