The Tumor Suppressor Adenomatous Polyposis Coli (apc) Is Required for Neural Crest-Dependent Craniofacial Development in Zebrafish
Abstract
:1. Introduction
2. Methods
2.1. Zebrafish Husbandry and Methods
2.2. RNA Isolation, RT-PCR, and Sequencing
2.3. Quantification of Alternative Spliced and Differentially Expressed Genes
2.4. Statistical Analysis and Downstream Bioinformatics
3. Results
3.1. The Tumor Suppressor Apc Is Required for the CNC Contribution to Craniofacial Structures
3.2. Increased Expression of Cytokines and Wnt Target Genes in apcmcr/mcr Larvae
3.3. Increased Expression of Complement c3 in apcmcr/mcr Larvae
3.4. An Oncogenic apc Mutation Alters mRNA Splicing
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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RT-qPCR | Forward | Reverse |
---|---|---|
CCL19 | CCCCATTGCAGCTACTGTATTC | AGGTGTTTTTCTCTGGTGGGG |
IL-11a | CCGGTTCAAGTCTCTTCCAG | AGGTTTGCATGGAGCTGAGA |
IL-11b | CATCTTATCCAAGCTATCATCCAG | GATCTCGGGTGCTGTCTGTC |
LEPA | GGAACACATTGACGGGCAAA | ATGGGTTTGTCAGCGGGAAT |
CBX7a | TGCGGTGGAGTCAATAACGAA | CAGGTGCTGTACTTAGGCGA |
DKK1a | GTGGAGTTTGTCTGTCGTGC | AAGCTGACACACACCGTTGA |
DKK1b | CACCGCAGCAGCCTTTAATC | CGCGAGACTGGAAGCAAAAC |
NOTUM1a | CACCTGTAACGACGGGACTC | CCAGCCGCCCTCAAGAAATA |
LFT1 | ACGCACGAGTGAGCATCTAC | CGTGAATGGGAATCAACCTGG |
SALL1b | CTTCAGGGAGATAACCCGGC | CCATCGTATGATCGAGGAGAACA |
SOCS3a | AAGCAGGGAAGACAAGAGCC | AGAGCTGGTCAAAAGAGCCTAT |
GSC | CCTACAGGTTATGACAGCGCC | GACAAGGTGCCCACGTTCAT |
EMILIN3a | GGCACAAGAACCACTGTGCAT | CCAAGCACACTTCATTGCCT |
GAPDH | GTGGAGTCTACTGGTGTCTTC | GTGCAGGAGGCATTGCTTACA |
C3A.1 | GACGCCCAACTTGAAACCAC | GCAACCTCAGGGATGGCATA |
C3A.6 | ACCAGGAATGCCCTTCAGTG | ACCTTTGACTCCTCCGGGAT |
RT-qPCR for splice forms: | ||
SEMA3fa_ALL | ACTCAACAGCGTCTCAGCTT | TACTGGTCGGTCCTCATTGC |
SEMA3fa_Long splice form | ACAACCCCATCTGCACCTATG | TCTGGTGAGGCACTAGGGT |
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Liu, X.; Jones, W.D.; Quesnel-Vallières, M.; Devadiga, S.A.; Lorent, K.; Valvezan, A.J.; Myers, R.L.; Li, N.; Lengner, C.J.; Barash, Y.; et al. The Tumor Suppressor Adenomatous Polyposis Coli (apc) Is Required for Neural Crest-Dependent Craniofacial Development in Zebrafish. J. Dev. Biol. 2023, 11, 29. https://doi.org/10.3390/jdb11030029
Liu X, Jones WD, Quesnel-Vallières M, Devadiga SA, Lorent K, Valvezan AJ, Myers RL, Li N, Lengner CJ, Barash Y, et al. The Tumor Suppressor Adenomatous Polyposis Coli (apc) Is Required for Neural Crest-Dependent Craniofacial Development in Zebrafish. Journal of Developmental Biology. 2023; 11(3):29. https://doi.org/10.3390/jdb11030029
Chicago/Turabian StyleLiu, Xiaolei, William D. Jones, Mathieu Quesnel-Vallières, Sudhish A. Devadiga, Kristin Lorent, Alexander J. Valvezan, Rebecca L. Myers, Ning Li, Christopher J. Lengner, Yoseph Barash, and et al. 2023. "The Tumor Suppressor Adenomatous Polyposis Coli (apc) Is Required for Neural Crest-Dependent Craniofacial Development in Zebrafish" Journal of Developmental Biology 11, no. 3: 29. https://doi.org/10.3390/jdb11030029