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Open AccessArticle

Evaluation of Acute and Subacute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats

1
Department of Pharmacology, Faculty of Pharmacy, IFTM University, Moradabad 244102, India
2
Department of Pharmacy, M.J.P. Rohilkhand University, Bareilly 243006, India
*
Author to whom correspondence should be addressed.
Academic Editor: Gernot A. Eller
Sci. Pharm. 2017, 85(3), 29; https://doi.org/10.3390/scipharm85030029
Received: 18 July 2017 / Revised: 7 August 2017 / Accepted: 7 August 2017 / Published: 21 August 2017
The objective of this study is to evaluate the acute and subacute toxicity of the ethanolic extract of Marsdenia tenacissima (MTE) leaves (family: Asclepiadaceae) in albino rats. The acute toxicity was performed where the limit dose of 5000 mg/kg body weight used. Observations were made and recorded for 24 h, and once daily further for a period of 14 days. The rats were weighed and various observations, like mortality, behavior, injury, or any signs of illness were conducted once daily during the period. For subacute study, four groups of 10 animals (female rats) received 10% Tween 20 in distilled water (control), and 250, 500, and 1000 mg/kg of freshly-prepared extracts, respectively, every 24 h orally for 28 days. At the end of each study, hematological analysis and biochemical parameters were evaluated. Histopathological examination of vital organs of the animals were taken for gross findings, compared to controls. There was no significant difference (p > 0.05) observed in the relative organs, body weights, hematological, biochemical parameters, and gross abnormalities, compared to the control. No mortality was recorded. Therefore, analysis of results may lead to the conclusion that the medium-term oral administration of the MTE leaves for 28 days does not cause toxicity. View Full-Text
Keywords: acute toxicity; biochemical analysis; hematological parameters; Marsdenia tenacissima; subacute toxicity; histopathology acute toxicity; biochemical analysis; hematological parameters; Marsdenia tenacissima; subacute toxicity; histopathology
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Porwal, M.; Khan, N.A.; Maheshwari, K.K. Evaluation of Acute and Subacute Oral Toxicity Induced by Ethanolic Extract of Marsdenia tenacissima Leaves in Experimental Rats. Sci. Pharm. 2017, 85, 29.

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