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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2015). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Sci. Pharm. 2012, 80(4), 977-986; (registering DOI)

A Validated Stability-Indicating HPLC Method for Routine Analysis of an Injectable Lincomycin and Spectinomycin Formulation

Department of Pharmacy, An-Najah National University, Nablus, Palestine
Advanced Veterinary Company, Ramallah, Palestine
Division of Chemistry & Environmental Science, School of Science and the Environment, Manchester Metropolitan University, Manchester, United Kingdom
Author to whom correspondence should be addressed.
Received: 16 July 2012 / Accepted: 10 September 2012 / Published: 10 September 2012
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Lincomycin and spectinomycin combination therapy is widely used in veterinary medicine for the treatment of gastrointestinal and respiratory infections caused by lincomycin- and spectinomycin-sensitive microorganisms. A simple, reverse phase HPLC method for the analysis of samples of an injectable lincomycin and spectinomycin preparation containing a mixture of inactive excipients has been developed. The HPLC was carried out using the RP-C18 (250 mm × 4.0 mm, 5 μm) column, with the gradient mobile phase consisting of an acetonitrile and phosphate buffer at pH 6; the flow rate of 1 mL/min and ultraviolet detection at 220 nm. This method was validated in accordance with both FDA and ICH guidelines and showed good linearity, accuracy, precision, selectivity, and system suitability results within the acceptance criteria. A stability-indicating study was also carried out and indicated that this method can also be used for purity and degradation evaluation of these formulations.
Keywords: Stability-Indicating; RP-HPLC; Lincomycin; Spectinomycin Stability-Indicating; RP-HPLC; Lincomycin; Spectinomycin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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ABUALHASAN, M.N.; BATRAWI, N.; SUTCLIFFE, O.B.; ZAID, A.N. A Validated Stability-Indicating HPLC Method for Routine Analysis of an Injectable Lincomycin and Spectinomycin Formulation. Sci. Pharm. 2012, 80, 977-986.

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