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Sci. Pharm.
Volume 80
Issue 4
10.3797/scipharm.1207-07
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Article

New Stability-Indicating RP-UFLC Method for Determination of Trospium Chloride in Tablet Dosage Form

by
Sagar Suman PANDA
*,
Bera V. V. RAVI KUMAR
,
Ganeswar MOHANTA
,
Rabisankar DASH
and
Pinkal Kumar PATEL
Department of Pharmaceutical Analysis and Quality Assurance, Roland Institute of Pharmaceutical Sciences, Khodasingi, 760010, Berhampur (Odisha), India
*
Author to whom correspondence should be addressed.
Sci. Pharm. 2012, 80(4), 955-964; https://doi.org/10.3797/scipharm.1207-07
Submission received: 12 July 2012 / Accepted: 31 August 2012 / Published: 31 August 2012
Versions Notes

Abstract

A simple, precise, and accurate isocratic RP-UFLC stability-indicating assay method has been developed to determine trospium chloride in tablet dosage form. Isocratic separation was achieved on an Enable-C18G (250 mm × 4.6 mm i.d., particle size 5 μm) column at room temperature, the mobile phase consisted of acetonitrile:0.01M TBAHS (50:50, v/v) at a flow rate of 1.0 ml/min, the injection volume was 20 μl, and PDA detection was carried out at 215 nm. The drug was subjected to acid and alkali hydrolysis, oxidation, photolysis, and heat as stress conditions. The method was validated for specificity, linearity, precision, accuracy, robustness, and system suitability. The method was linear in the drug concentration range of 10–300 μg/ml with the correlation coefficient being 0.999. The RSD for repeatability and intermediate precision was well below 2%. The mean recoveries were between 100.52–101.68% for trospium chloride.
Keywords: Trospium chloride; UFLC; Forced degradation; TBAHS Trospium chloride; UFLC; Forced degradation; TBAHS

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MDPI and ACS Style

PANDA, S.S.; RAVI KUMAR, B.V.V.; MOHANTA, G.; DASH, R.; PATEL, P.K. New Stability-Indicating RP-UFLC Method for Determination of Trospium Chloride in Tablet Dosage Form. Sci. Pharm. 2012, 80, 955-964. https://doi.org/10.3797/scipharm.1207-07

AMA Style

PANDA SS, RAVI KUMAR BVV, MOHANTA G, DASH R, PATEL PK. New Stability-Indicating RP-UFLC Method for Determination of Trospium Chloride in Tablet Dosage Form. Scientia Pharmaceutica. 2012; 80(4):955-964. https://doi.org/10.3797/scipharm.1207-07

Chicago/Turabian Style

PANDA, Sagar Suman, Bera V. V. RAVI KUMAR, Ganeswar MOHANTA, Rabisankar DASH, and Pinkal Kumar PATEL. 2012. "New Stability-Indicating RP-UFLC Method for Determination of Trospium Chloride in Tablet Dosage Form" Scientia Pharmaceutica 80, no. 4: 955-964. https://doi.org/10.3797/scipharm.1207-07

APA Style

PANDA, S. S., RAVI KUMAR, B. V. V., MOHANTA, G., DASH, R., & PATEL, P. K. (2012). New Stability-Indicating RP-UFLC Method for Determination of Trospium Chloride in Tablet Dosage Form. Scientia Pharmaceutica, 80(4), 955-964. https://doi.org/10.3797/scipharm.1207-07

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