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Article

Radiation-Inactivated Acinetobacter baumannii Vaccine Candidates

1
Biological Mimetics, Inc., 124 Byte Drive, Frederick, MD 21702, USA
2
Wound Infections Department, Bacterial Diseases Branch, Center for Infectious Disease Research, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA
3
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA
4
Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA
5
Defense Threat Reduction Agency, Ft. Belvoir, VA 22060, USA
*
Author to whom correspondence should be addressed.
The authors contributed equally to this work.
Academic Editor: Vasso Apostolopoulos
Vaccines 2021, 9(2), 96; https://doi.org/10.3390/vaccines9020096
Received: 29 December 2020 / Revised: 22 January 2021 / Accepted: 23 January 2021 / Published: 27 January 2021
(This article belongs to the Special Issue Vaccines for Infectious and Chronic Diseases)
Acinetobacter baumannii is a bacterial pathogen that is often multidrug-resistant (MDR) and causes a range of life-threatening illnesses, including pneumonia, septicemia, and wound infections. Some antibiotic treatments can reduce mortality if dosed early enough before an infection progresses, but there are few other treatment options when it comes to MDR-infection. Although several prophylactic strategies have been assessed, no vaccine candidates have advanced to clinical trials or have been approved. Herein, we rapidly produced protective whole-cell immunogens from planktonic and biofilm-like cultures of A. baumannii, strain AB5075 grown using a variety of methods. After selecting a panel of five cultures based on distinct protein profiles, replicative activity was extinguished by exposure to 10 kGy gamma radiation in the presence of a Deinococcus antioxidant complex composed of manganous (Mn2+) ions, a decapeptide, and orthophosphate. Mn2+ antioxidants prevent hydroxylation and carbonylation of irradiated proteins, but do not protect nucleic acids, yielding replication-deficient immunogenic A. baumannii vaccine candidates. Mice were immunized and boosted twice with 1.0 × 107 irradiated bacterial cells and then challenged intranasally with AB5075 using two mouse models. Planktonic cultures grown for 16 h in rich media and biofilm cultures grown in static cultures underneath minimal (M9) media stimulated immunity that led to 80–100% protection. View Full-Text
Keywords: A. baumannii; vaccine; mouse; whole-cell; irradiated; protective; MDP; inactivated; pulmonary; Deinococcus A. baumannii; vaccine; mouse; whole-cell; irradiated; protective; MDP; inactivated; pulmonary; Deinococcus
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MDPI and ACS Style

Dollery, S.J.; Zurawski, D.V.; Gaidamakova, E.K.; Matrosova, V.Y.; Tobin, J.K.; Wiggins, T.J.; Bushnell, R.V.; MacLeod, D.A.; Alamneh, Y.A.; Abu-Taleb, R.; Escatte, M.G.; Meeks, H.N.; Daly, M.J.; Tobin, G.J. Radiation-Inactivated Acinetobacter baumannii Vaccine Candidates. Vaccines 2021, 9, 96. https://doi.org/10.3390/vaccines9020096

AMA Style

Dollery SJ, Zurawski DV, Gaidamakova EK, Matrosova VY, Tobin JK, Wiggins TJ, Bushnell RV, MacLeod DA, Alamneh YA, Abu-Taleb R, Escatte MG, Meeks HN, Daly MJ, Tobin GJ. Radiation-Inactivated Acinetobacter baumannii Vaccine Candidates. Vaccines. 2021; 9(2):96. https://doi.org/10.3390/vaccines9020096

Chicago/Turabian Style

Dollery, Stephen J., Daniel V. Zurawski, Elena K. Gaidamakova, Vera Y. Matrosova, John K. Tobin, Taralyn J. Wiggins, Ruth V. Bushnell, David A. MacLeod, Yonas A. Alamneh, Rania Abu-Taleb, Mariel G. Escatte, Heather N. Meeks, Michael J. Daly, and Gregory J. Tobin 2021. "Radiation-Inactivated Acinetobacter baumannii Vaccine Candidates" Vaccines 9, no. 2: 96. https://doi.org/10.3390/vaccines9020096

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