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Article

Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious Bronchitis Virus

College of Animal Science and Technology, Guangxi University, Nanning 530004, China
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Authors to whom correspondence should be addressed.
Academic Editor: Vita Golubovskaya
Vaccines 2021, 9(2), 146; https://doi.org/10.3390/vaccines9020146
Received: 27 December 2020 / Revised: 27 January 2021 / Accepted: 9 February 2021 / Published: 11 February 2021
(This article belongs to the Special Issue Advances in Vaccine Development and Immunotherapies)
Infectious bronchitis virus (IBV) poses massive economic losses in the global poultry industry. Here, we firstly report the construction and immunogenicity comparison of virus-like particles (VLPs) carrying the S, M and E proteins (SME-VLPs); VLPs carrying the S and M proteins (SM-VLPs); and VLPs carrying the M and E proteins (ME-VLPs) from the dominant serotype representative strain GX-YL5 in China. The neutralizing antibody response induced by the SME-VLPs was similar to that induced by the inactivated oil vaccine (OEV) of GX-YL5, and higher than those induced by the SM-VLPs, ME-VLPs and commercial live vaccine H120. More importantly, the SME-VLPs elicited higher percentages of CD4+ and CD8+ T lymphocytes than the SM-VLPs, ME-VLPs and OEV of GX-YL5. Compared with the OEV of GX-YL5, higher levels of IL-4 and IFN-γ were also induced by the SME-VLPs. Moreover, the mucosal immune response (sIgA) induced by the SME-VLPs in the tear and oral swabs was comparable to that induced by the H120 vaccine and higher than that induced by the OEV of GX-YL5. In the challenge experiment, the SME-VLPs resulted in significantly lower viral RNA levels in the trachea and higher protection scores than the OEV of GX-YL5 and H120 vaccines, and induced comparable viral RNA levels in the kidneys, and tear and oral swabs to the OEV of GX-YL5. In summary, among the three VLPs, the SME-VLPs carrying the S, M and E proteins of IBV could stimulate the strongest humoral, cellular and mucosal immune responses and provide effective protection, indicating that it would be an attractive vaccine candidate for IB. View Full-Text
Keywords: infectious bronchitis virus; virus-like particles; construction; immunogenicity infectious bronchitis virus; virus-like particles; construction; immunogenicity
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MDPI and ACS Style

Zhang, Y.; Yuan, Y.; Zhang, L.-H.; Zhu, D.; Wang, L.; Wei, L.-P.; Fan, W.-S.; Zhao, C.-R.; Su, Y.-J.; Liao, J.-Q.; Yong, L.; Wei, T.-C.; Wei, P.; Mo, M.-L. Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious Bronchitis Virus. Vaccines 2021, 9, 146. https://doi.org/10.3390/vaccines9020146

AMA Style

Zhang Y, Yuan Y, Zhang L-H, Zhu D, Wang L, Wei L-P, Fan W-S, Zhao C-R, Su Y-J, Liao J-Q, Yong L, Wei T-C, Wei P, Mo M-L. Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious Bronchitis Virus. Vaccines. 2021; 9(2):146. https://doi.org/10.3390/vaccines9020146

Chicago/Turabian Style

Zhang, Yu, Yuan Yuan, Li-Hua Zhang, Dan Zhu, Lu Wang, Lan-Ping Wei, Wen-Sheng Fan, Chang-Run Zhao, Yan-Jing Su, Jian-Qi Liao, Lu Yong, Tian-Chao Wei, Ping Wei, and Mei-Lan Mo. 2021. "Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious Bronchitis Virus" Vaccines 9, no. 2: 146. https://doi.org/10.3390/vaccines9020146

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