Proteomic as an Exploratory Approach to Develop Vaccines Against Tick-Borne Diseases Using Lyme Borreliosis as a Test Case
Abstract
:1. Introduction
2. Material and Methods
2.1. Mouse Infection and Bacterial Strains
2.2. Quantification of B. burgdorferi DNA by PCR in Mouse Skin
2.3. Proteomics
2.4. In Silico Evaluation of Protein Polymorphism Among Borrelia burgdorferi Coding DNA Sequences
2.5. Ethics
2.6. Statistical Analyses
3. Results
3.1. Efficacy of Infection and Kinetics of Bacterial Multiplication in the Mouse Skin for the Three B. burgdorferi ss Strains
3.2. Detection of Borrelia Proteins on Day 7 by Proteomics
3.3. Peptidic Polymorphism of the Selected Coding DNA Sequences Among B. burgdorferi ss Genomes
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Days Post-Inoculation (dpi) | Strains | Positive PCR Amplification | |
---|---|---|---|
Positive Reactions/No. of Tested Animals | Rate of Positivity (CI 95%) | ||
3 dpi | 297 | 14/19 | 74% (49–91) |
IBS 19 | 6/11 | 55% (23–83) | |
BL 642 | 4/11 | 36% (11–69) | |
5 dpi | 297 | 16/17 | 94% (71–100) |
IBS 19 | 8/8 | 100% (63–100) | |
BL 642 | 6/7 | 86% (42–100) | |
7 dpi | 297 | 15/15 | 100% (78–100) |
IBS 19 | 10/10 | 100% (69–100) | |
BL 642 | 6/6 | 100% (54–100) | |
15 dpi | 297 | 6/13 | 46% (19–75) |
IBS 19 | 3/7 | 43% (10–82) | |
BL 642 | 3/7 | 43% (10–82) |
Protein Name | Accession Number (Borrelia burgdorferi B31) | Accession Number (Borrelia burgdorferi Pko) | Infection via Syringe Inoculation Borrelia burgdorferi Strain (Spirochete Density Flagellin/104gapdh) | Infection via Tick Bite (Spirochete Density Flagellin/104gapdh) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
297c4 (192) | 297c4 (106) | 297n (346) | IBS19 (85) | IBS19 (136) | IBS19 (166) | BL642 (157) | BL642 (227) | BL642 (268) | B. Afzelii NE4049 (66) | Field-Collected Ticks (534) | |||
Flagellin | gi|120230 | gi|111114970 | 7 | 8 | 7 | 2 | 2 | 3 | 3 | 2 | 4 | 8 | |
Osp C | gi|3914248 | gi|111074137 | 3 | 2 | 3 | 1 | 2 | 2 | 2 | 1 | 1 | 4 | 1 |
GAPDH | gi|238828321 gi|3915702 | 1 | 1 | 1 | 1 | 1 | |||||||
Glycosaminoglycan binding Protein (Bgp) | gi|15594933 | 1 | 1 | 1 | |||||||||
GroEL | gi|229553917 | gi|123145654 | 1 | 1 | 2 | ||||||||
p66 | gi|15594948 | 1 | |||||||||||
Fructose-bisphosphate aldolase | gi|6686370 | gi|111115272 | 1 | ||||||||||
DbpA | gi|17373807 | 1 | |||||||||||
DbpB | gi|327507700 | 2 | |||||||||||
Elongation factor 4 | gi|15594434 gi|6016495 | 1 | |||||||||||
Elongation factor Tu | gi|1706598 | gi|123341337 | 1 | 1 | 1 | 2 | |||||||
Enolase | gi|3913583 | 1 | |||||||||||
Lipoprotein BbA36 | gi|365823350 | 2 | |||||||||||
L-lactate dehydrogenase | gi|15594433 gi|17367476 | 1 | |||||||||||
hypothetical protein BB_0363 | gi|15594708 | 1 | |||||||||||
hypothetical protein BB_0563 | gi|365992369 | 1 | 1 | ||||||||||
hypothetical protein BB_F14 | gi|365823340 | 1 | 1 | ||||||||||
hypothetical protein BB_J48 | gi|364556751 | 1 | |||||||||||
hypothetical protein BAPKO_4515 | gi|117621815 | 1 | |||||||||||
hypothetical protein BAPKO_0028 | gi|111114851 | 1 | |||||||||||
hypothetical protein BAPKO_0593 | gi|111115391 | 1 | |||||||||||
hypothetical protein BAPKO_2500 | gi|117621647 | 1 | |||||||||||
50S ribosomal protein L7/L12 | gi|123046997 | 1 | |||||||||||
30S ribosomal protein S16 | gi|123145645 | 1 | 1 | ||||||||||
Neutrophil activating protein | gi|111115523 | 2 | |||||||||||
Phosphoglyceromutase | gi|123145651 | 1 | |||||||||||
Flagellar filament outer layer protein | gi|111115501 | 1 | |||||||||||
ATP-dependent protease | gi|111115078 | 1 | 1 | ||||||||||
Periplasmic oligopeptide-binding protein | gi|111115153 | 1 | |||||||||||
Chemotaxis protein methyltransferase | gi|111114863 | 1 | |||||||||||
Number of Borrelia proteins | 9 | 6 | 7 | 2 | 2 | 2 | 3 | 6 | 5 | 7 | 15 | ||
Number of mouse proteins | 1831 | 1791 | 1824 | 1486 | 1617 | 1550 | 1702 | 1351 | 1426 | 5916 | 5926 |
Protein (Length) | Protein Accession Number (B31) | Gene Accession Number (B31) | CDS Location | Peptidic Variability Among the CDS of 15 B. burgdorferi Sensu Stricto Strains |
---|---|---|---|---|
FlaB (336 aa) | SwissProt P11089 | Genbank BB_0147 | chromosome | 100% S./100% Id.: 15/15 strains |
GAPDH (335 aa) | SwissProt P46795 | Genbank BB_0057 | chromosome | 100% S./100% Id.: 14/15 strains 100% S./99% Id.: 1/15 strain |
GroEL (545 aa) | SwissProt P0C923 | Genbank BB_0649 | chromosome | 100% S./100% Id.: 15/15 strains 1 supplementary aa in 1 strain |
Enolase (433 aa) | SwissProt O51312 | Genbank BB_0337 | chromosome | 100% S./100% Id.: 12/15 strains 100% S./99% Id.: 2/15 strains Truncated peptidic CDS in the 29805 strain (from 219th to 273th aa) |
Lipoprotein BbA36 (212 aa) | TrEmbl O50929 | Genbank BB_A36 | lp54 plasmid(13/15 strains) | 100% S./100% Id.: 2/13 strains 100% S./99% Id.: 2/13 strains 97–99% S./97–99% Id.: 9/13 strains Not found in 2 WGS without sequenced lp54 plasmid 8 first aa not annotated in the 12 strains other than B31 1 supplementary Asparagin between the 80th and 81th residue of the reference sequence in 6/13 strains |
Outer surface protein C (210 aa) | SwissProt Q07337 | Genbank BB_B19 | cp26 plasmid(13/15 strains) | 100% S./100% Id.: 1/13 strains 82–88% S./74–81% Id.: 12/13 strains Not found in 2 WGS without sequenced cp26 plasmid |
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Talagrand-Reboul, E.; Westermann, B.; Raess, M.A.; Schnell, G.; Cantero, P.; Barthel, C.; Ehret-Sabatier, L.; Jaulhac, B.; Boulanger, N. Proteomic as an Exploratory Approach to Develop Vaccines Against Tick-Borne Diseases Using Lyme Borreliosis as a Test Case. Vaccines 2020, 8, 463. https://doi.org/10.3390/vaccines8030463
Talagrand-Reboul E, Westermann B, Raess MA, Schnell G, Cantero P, Barthel C, Ehret-Sabatier L, Jaulhac B, Boulanger N. Proteomic as an Exploratory Approach to Develop Vaccines Against Tick-Borne Diseases Using Lyme Borreliosis as a Test Case. Vaccines. 2020; 8(3):463. https://doi.org/10.3390/vaccines8030463
Chicago/Turabian StyleTalagrand-Reboul, Emilie, Benoit Westermann, Matthieu A. Raess, Gilles Schnell, Paola Cantero, Cathy Barthel, Laurence Ehret-Sabatier, Benoit Jaulhac, and Nathalie Boulanger. 2020. "Proteomic as an Exploratory Approach to Develop Vaccines Against Tick-Borne Diseases Using Lyme Borreliosis as a Test Case" Vaccines 8, no. 3: 463. https://doi.org/10.3390/vaccines8030463