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Vaccines 2015, 3(3), 751-770;

New Data on Vaccine Antigen Deficient Bordetella pertussis Isolates

Molecular Prevention and Therapy of Human Diseases, Institut Pasteur, 25 rue du Dr Roux, Paris 75015, France
URAS-CNRS 3012, Paris 75015, France
Present address: Center for Vaccine Development, School of Medicine, University of Maryland Baltimore, 685 West Baltimore Street, Room480, Baltimore, MD 21201, USA.
Present address: Department of Computational Biology, Research and Innovation Centre, Fondazione Edmund Mach, via E. Mach 1, San Michele all'Adige 38010, Italy.
Present address: Centre for Integrative Biology, University of Trento, via Sommarive 9, 38123 Trento, Italy.
Present address: Institut Pasteur, Unit
Author to whom correspondence should be addressed.
Academic Editor: Diane M. Harper
Received: 13 July 2015 / Revised: 25 August 2015 / Accepted: 2 September 2015 / Published: 14 September 2015
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Evolution of Bordetella pertussis is driven by natural and vaccine pressures. Isolates circulating in regions with high vaccination coverage present multiple allelic and antigenic variations as compared to isolates collected before introduction of vaccination. Furthermore, during the last epidemics reported in regions using pertussis acellular vaccines, isolates deficient for vaccine antigens, such as pertactin (PRN), were reported to reach high proportions of circulating isolates. More sporadic filamentous hemagglutinin (FHA) or pertussis toxin (PT) deficient isolates were also collected. The whole genome of some recent French isolates, deficient or non-deficient in vaccine antigens, were analyzed. Transcription profiles of the expression of the main virulence factors were also compared. The invasive phenotype in an in vitro human tracheal epithelial (HTE) cell model of infection was evaluated. Our genomic analysis focused on SNPs related to virulence genes known to be more likely to present allelic polymorphism. Transcriptomic data indicated that isolates circulating since the introduction of pertussis vaccines present lower transcription levels of the main virulence genes than the isolates of the pre-vaccine era. Furthermore, isolates not producing FHA present significantly higher expression levels of the entire set of genes tested. Finally, we observed that recent isolates are more invasive in HTE cells when compared to the reference strain, but no multiplication occurs within cells. View Full-Text
Keywords: Bordetella pertussis; pertactin; filamentous hemagglutinin; vaccine antigen production deficience Bordetella pertussis; pertactin; filamentous hemagglutinin; vaccine antigen production deficience

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Bouchez, V.; Hegerle, N.; Strati, F.; Njamkepo, E.; Guiso, N. New Data on Vaccine Antigen Deficient Bordetella pertussis Isolates. Vaccines 2015, 3, 751-770.

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