Next Article in Journal
Meningitis in a School-Aged Child due to Haemophilus influenzae Type E during the Post-Conjugate Vaccine Era—Monroe County, NY, 2011
Next Article in Special Issue
DNA/MVA Vaccines for HIV/AIDS
Previous Article in Journal
Development of Streptococcus pneumoniae Vaccines Using Live Vectors
Previous Article in Special Issue
Pilot Study on the Use of DNA Priming Immunization to Enhance Y. pestis LcrV-Specific B Cell Responses Elicited by a Recombinant LcrV Protein Vaccine
Article Menu

Export Article

Open AccessReview

Recent Developments in Preclinical DNA Vaccination

Department of Molecular Biodefense Research, Yokohama City University Graduate School of Medicine, Yokohama, Kanagawa Prefecture 236-0004, Japan
Choju Medical Institute, Toyohashi, Aichi Prefecture 441-8124, Japan
Vaccine Institute, Yokohama, Kanagawa Prefecture 2235-0045, Japan
Author to whom correspondence should be addressed.
Vaccines 2014, 2(1), 89-106;
Received: 15 October 2013 / Revised: 22 November 2013 / Accepted: 26 November 2013 / Published: 13 January 2014
(This article belongs to the Special Issue DNA Vaccines)
PDF [381 KB, uploaded 13 January 2014]


The advantages of genetic immunization of the new vaccine using plasmid DNAs are multifold. For example, it is easy to generate plasmid DNAs, increase their dose during the manufacturing process, and sterilize them. Furthermore, they can be stored for a long period of time upon stabilization, and their protein encoding sequences can be easily modified by employing various DNA-manipulation techniques. Although DNA vaccinations strongly increase Th1-mediated immune responses in animals, several problems persist. One is about their weak immunogenicity in humans. To overcome this problem, various genetic adjuvants, electroporation, and prime-boost methods have been developed preclinically, which are reviewed here. View Full-Text
Keywords: DNA vaccine; genetic adjuvants; electroporation DNA vaccine; genetic adjuvants; electroporation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

MDPI and ACS Style

Okuda, K.; Wada, Y.; Shimada, M. Recent Developments in Preclinical DNA Vaccination. Vaccines 2014, 2, 89-106.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Vaccines EISSN 2076-393X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top