Hercynine, Ergothioneine and Redox State in Stallion’s Seminal Plasma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals, Semen and Seminal Plasma Samplings
2.2. Quantification of Ergothioneine (ERT) in Seminal Plasma
2.3. Quantification of Hercynine (ERY) in Seminal Plasma
2.4. Quantification of Glutathione (GSH) and Cysteine (Cys) in Seminal Plasma
2.5. Quantification of Free Thiol Groups in Seminal Plasma (DTNB)
2.6. Trolox Equivalent Antioxidant Capacity Assay (TEAC)
2.7. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Withdrawal #1 | Withdrawal #2 | Withdrawal #3 | Withdrawal #4 | |
---|---|---|---|---|
ERT µmol/L ± SD | 234.59 ± 156.13 | 310.61 ± 225.76 | 369.91 ± 260.70 | 363.01 ± 232.34 |
ERY nmol/L (IQR) | 6.31 (16.10) | 6.41 (11.21) | 5.33 (15.43) | 11.54 (12.66) |
GSH µmol/L (IQR) | 1.71 (1.29) | 1.28 (0.99) | 1.11 (0.44) | 0.98 (1.05) |
Cys µmol/L (IQR) | 40.23 (34.08) | 32.47 (22.09) | 25.38 (33.09) | 29.90 (35.65) |
DTNB µmol/L (IQR) | 3.36 (1.24) | 4.20 (3.01) | 3.18 (2.09) | 3.63 (1.27) |
TEAC μmolTE/100 g (IQR) | 7.46 (1.36) | 6.33 (2.97) | 8.19 (5.98) | 6.10 (4.59) |
Sperm Volume mL ± SD | 25 ± 12 | 32 ± 13 | 30 ± 11 | 33 ± 14 |
Sperm Concentration million per mL (IQR) | 360 (288) | 358 (169) | 415 (231) | 402 (169) |
Total Motility % (IQR) | 83 (10) | 93 (17) | 90 (15) | 91 (5) |
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Sotgia, S.; Taras, A.; Zinellu, A.; Cherchi, R.; Mangoni, A.A.; Carru, C.; Bogliolo, L. Hercynine, Ergothioneine and Redox State in Stallion’s Seminal Plasma. Antioxidants 2020, 9, 855. https://doi.org/10.3390/antiox9090855
Sotgia S, Taras A, Zinellu A, Cherchi R, Mangoni AA, Carru C, Bogliolo L. Hercynine, Ergothioneine and Redox State in Stallion’s Seminal Plasma. Antioxidants. 2020; 9(9):855. https://doi.org/10.3390/antiox9090855
Chicago/Turabian StyleSotgia, Salvatore, Andrea Taras, Angelo Zinellu, Raffaele Cherchi, Arduino A Mangoni, Ciriaco Carru, and Luisa Bogliolo. 2020. "Hercynine, Ergothioneine and Redox State in Stallion’s Seminal Plasma" Antioxidants 9, no. 9: 855. https://doi.org/10.3390/antiox9090855