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Article

Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells

1
Retinal Pathologies and New Therapies Group, Experimental Ophthalmology Laboratory, Department of Ophthalmology, Clinica Universidad de Navarra, 31008 Pamplona, Spain
2
Navarra Institute for Health Research, IdiSNA, 31008 Pamplona, Spain
3
Red Temática de Investigación Cooperativa Sanitaria en Enfermedades Oculares (Oftared), 31008 Pamplona, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
These authors contributed equally to this work.
Antioxidants 2020, 9(9), 838; https://doi.org/10.3390/antiox9090838
Received: 31 July 2020 / Revised: 2 September 2020 / Accepted: 4 September 2020 / Published: 8 September 2020
(This article belongs to the Special Issue Oxidative Stress in Diabetic Retinopathy)
Diabetic retinopathy is a vision-threatening microvascular complication of diabetes and is one of the leading causes of blindness. Oxidative stress and inflammation play a major role in its pathogenesis, and new therapies counteracting these contributors could be of great interest. In the current study, we investigated the role of vitamin D against oxidative stress and inflammation in human retinal pigment epithelium (RPE) and human retinal endothelial cell lines. We demonstrate that vitamin D effectively counteracts the oxidative stress induced by hydrogen peroxide (H2O2). In addition, the increased levels of proinflammatory proteins such as Interleukin (IL)-6, IL-8, Monocyte chemoattractant protein (MCP)-1, Interferon (IFN)-γ, and tumor necrosis factor (TNF)-α triggered by lipopolysaccharide (LPS) exposure were significantly decreased by vitamin D addition. Interestingly, the increased IL-18 only decreased by vitamin D addition in endothelial cells but not in RPE cells, suggesting a main antiangiogenic role under inflammatory conditions. Moreover, H2O2 and LPS induced the alteration and morphological damage of tight junctions in adult retinal pigment epithelium (ARPE-19) cells that were restored under oxidative and inflammatory conditions by the addition of vitamin D to the media. In conclusion, our data suggest that vitamin D could protect the retina by enhancing antioxidant defense and through exhibiting anti-inflammatory properties. View Full-Text
Keywords: vitamin D; oxidative stress; inflammation; diabetic retinopathy vitamin D; oxidative stress; inflammation; diabetic retinopathy
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MDPI and ACS Style

Fernandez-Robredo, P.; González-Zamora, J.; Recalde, S.; Bilbao-Malavé, V.; Bezunartea, J.; Hernandez, M.; Garcia-Layana, A. Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells. Antioxidants 2020, 9, 838. https://doi.org/10.3390/antiox9090838

AMA Style

Fernandez-Robredo P, González-Zamora J, Recalde S, Bilbao-Malavé V, Bezunartea J, Hernandez M, Garcia-Layana A. Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells. Antioxidants. 2020; 9(9):838. https://doi.org/10.3390/antiox9090838

Chicago/Turabian Style

Fernandez-Robredo, Patricia; González-Zamora, Jorge; Recalde, Sergio; Bilbao-Malavé, Valentina; Bezunartea, Jaione; Hernandez, Maria; Garcia-Layana, Alfredo. 2020. "Vitamin D Protects against Oxidative Stress and Inflammation in Human Retinal Cells" Antioxidants 9, no. 9: 838. https://doi.org/10.3390/antiox9090838

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