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Aronia melanocarpa Fruit Bioactive Fraction Attenuates LPS-Induced Inflammatory Response in Human Bronchial Epithelial Cells

1
Department of Medicine, Graduate School, Kyung Hee University, Seoul 02453, Korea
2
Medical Science Research Institute, Kyung Hee University Medical Center, Seoul 02447, Korea
3
Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul 02447, Korea
4
Department of Clinical Pharmacology and Therapeutics, College of Medicine, Kyung Hee University, Seoul 02447, Korea
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(9), 816; https://doi.org/10.3390/antiox9090816
Received: 29 July 2020 / Revised: 26 August 2020 / Accepted: 31 August 2020 / Published: 2 September 2020
(This article belongs to the Section Health Outcomes of Antioxidants and Oxidative Stress)
To demonstrate the anti-inflammatory activity of Aronia melanocarpa fruit extract, human bronchial epithelial cells (BEAS-2B) were treated with lipopolysaccharide (LPS) and the effects of aronia bioactive fraction (ABF®), anthocyanin enriched extract from the fruit of A. melanocarpa, were evaluated. Following pretreatment with ABF® at 10–25 µg /mL, BEAS-2B cells were exposed to LPS and the expression of inflammatory mediators (tumor necrosis factor [TNF]-α, interleukin [IL]-6, IL-8, regulated upon activation, normal T cell expressed and presumably secreted [RANTES], IL-1β, cyclooxygenase-2 [COX-2], and inducible nitric oxide synthase [iNOS]) was analyzed. In LPS-stimulated BEAS-2B cells, ABF® pretreatment significantly decreased the mRNA expression of TNF-α, IL-6, IL-8, RANTES, IL-1β, and COX-2 at doses of 10 and 25 µg/mL. ABF® also attenuated the secretion of TNF- α, IL-6, IL-8, and RANTES protein, as demonstrated by enzyme linked immunosorbent assay. Western blot analyses revealed the decreased expression of COX-2 and iNOS following ABF® treatment. ROS production was decreased, and the cell cycle was arrested at the G0/G1 and S phases following ABF® pretreatment. Our results suggest that ABF® may have potential as a nutraceutical agent for the suppression of airway inflammation. View Full-Text
Keywords: BEAS-2B cells; aronia bioactive fraction (ABF®); anti-inflammatory effects; cytokine; reactive oxygen species BEAS-2B cells; aronia bioactive fraction (ABF®); anti-inflammatory effects; cytokine; reactive oxygen species
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MDPI and ACS Style

Jang, B.-K.; Lee, J.-W.; Choi, H.; Yim, S.-V. Aronia melanocarpa Fruit Bioactive Fraction Attenuates LPS-Induced Inflammatory Response in Human Bronchial Epithelial Cells. Antioxidants 2020, 9, 816. https://doi.org/10.3390/antiox9090816

AMA Style

Jang B-K, Lee J-W, Choi H, Yim S-V. Aronia melanocarpa Fruit Bioactive Fraction Attenuates LPS-Induced Inflammatory Response in Human Bronchial Epithelial Cells. Antioxidants. 2020; 9(9):816. https://doi.org/10.3390/antiox9090816

Chicago/Turabian Style

Jang, Bong-Keun, Jin-Woo Lee, Hyun Choi, and Sung-Vin Yim. 2020. "Aronia melanocarpa Fruit Bioactive Fraction Attenuates LPS-Induced Inflammatory Response in Human Bronchial Epithelial Cells" Antioxidants 9, no. 9: 816. https://doi.org/10.3390/antiox9090816

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