Silver Nanoparticles (AgNPs) as Enhancers of Everolimus and Radiotherapy Sensitivity on Clear Cell Renal Cell Carcinoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of AgNPs
2.2. Cell Culture and Treatment Conditions
2.3. AgNPs IC25 and IC50 Determination by Resazurin Assay
2.4. Determination of Cellular Localization of AgNPs by Transmission Electron Microscopy (TEM)
2.5. Cell Proliferation Assay—BrdU
2.6. Cell Cycle Analysis
2.7. Cell Apoptosis Assay
2.8. Detection of Intracellular Reactive Oxygen Species
2.9. Mitochondrial Membrane Potential Assay (TMRE)
2.10. HepG-2 Toxicity Evaluation—Resazurin Assay
2.11. Everolimus and AgNPs Combined Effect Evaluation
2.12. Irradiation
2.13. Statistical Analysis
3. Results
3.1. AgNPs’ Cytotoxic Potential Screening in RCC Cell Lines
3.2. The Phenotypic Effect of AgNPs in 786-O Cell Line
3.3. AgNPs Biocompatibility in HepG-2 Cell Line
3.4. AgNPs and Everolimus Combined Effect in 786-O Cell Line
3.5. AgNPs Effect as Sensitizers to Radiation in 786-O Cell Line
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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IC25 | IC50 |
---|---|
1712.15 µg/mL | 2629.63 µg/mL |
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Morais, M.; Machado, V.; Figueiredo, P.; Dias, F.; Craveiro, R.; Lencart, J.; Palmeira, C.; Mikkonen, K.S.; Teixeira, A.L.; Medeiros, R. Silver Nanoparticles (AgNPs) as Enhancers of Everolimus and Radiotherapy Sensitivity on Clear Cell Renal Cell Carcinoma. Antioxidants 2023, 12, 2051. https://doi.org/10.3390/antiox12122051
Morais M, Machado V, Figueiredo P, Dias F, Craveiro R, Lencart J, Palmeira C, Mikkonen KS, Teixeira AL, Medeiros R. Silver Nanoparticles (AgNPs) as Enhancers of Everolimus and Radiotherapy Sensitivity on Clear Cell Renal Cell Carcinoma. Antioxidants. 2023; 12(12):2051. https://doi.org/10.3390/antiox12122051
Chicago/Turabian StyleMorais, Mariana, Vera Machado, Patrícia Figueiredo, Francisca Dias, Rogéria Craveiro, Joana Lencart, Carlos Palmeira, Kirsi S. Mikkonen, Ana Luísa Teixeira, and Rui Medeiros. 2023. "Silver Nanoparticles (AgNPs) as Enhancers of Everolimus and Radiotherapy Sensitivity on Clear Cell Renal Cell Carcinoma" Antioxidants 12, no. 12: 2051. https://doi.org/10.3390/antiox12122051