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Article

The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation

1
Plant Physiology, University Bayreuth, Universitaetsstr. 30, 95440 Bayreuth, Germany
2
Department of Biochemistry and Physiology of Plants, University of Bielefeld, 33615 Bielefeld, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Bruce Morgan
Antioxidants 2021, 10(3), 401; https://doi.org/10.3390/antiox10030401
Received: 20 January 2021 / Revised: 23 February 2021 / Accepted: 4 March 2021 / Published: 7 March 2021
(This article belongs to the Special Issue Thiol-Based Redox Regulation of Cellular and Organismal Function)
The chloroplast primary metabolism is of central importance for plant growth and performance. Therefore, it is tightly regulated in order to adequately respond to multiple environmental conditions. A major fluctuation that plants experience each day is the change between day and night, i.e., the change between assimilation and dissimilation. Among other mechanisms, thioredoxin-mediated redox regulation is an important component of the regulation of plastid-localized metabolic enzymes. While assimilatory processes such as the Calvin–Benson cycle are activated under illumination, i.e., under reducing conditions, carbohydrate degradation is switched off during the day. Previous analyses have identified enzymes of the oxidative pentose phosphate pathway to be inactivated by reduction through thioredoxins. In this work, we present evidence that an enzyme of the plastidic glycolysis, the phosphofructokinase isoform AtPFK5, is also inactivated through reduction by thioredoxins, namely by thioredoxin-f. With the help of chemical oxidation, mutant analyses and further experiments, the highly conserved motif CXDXXC in AtPFK5 was identified as the target sequence for this regulatory mechanism. However, knocking out this isoform in plants had only very mild effects on plant growth and performance, indicating that the complex primary metabolism in plants can overcome a lack in AtPFK5 activity. View Full-Text
Keywords: phosphofructokinase; thioredoxin; Arabidopsis thaliana; redox regulation phosphofructokinase; thioredoxin; Arabidopsis thaliana; redox regulation
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MDPI and ACS Style

Hess, N.; Richter, S.; Liebthal, M.; Dietz, K.-J.; Mustroph, A. The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation. Antioxidants 2021, 10, 401. https://doi.org/10.3390/antiox10030401

AMA Style

Hess N, Richter S, Liebthal M, Dietz K-J, Mustroph A. The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation. Antioxidants. 2021; 10(3):401. https://doi.org/10.3390/antiox10030401

Chicago/Turabian Style

Hess, Natalia, Simon Richter, Michael Liebthal, Karl-Josef Dietz, and Angelika Mustroph. 2021. "The Phosphofructokinase Isoform AtPFK5 Is a Novel Target of Plastidic Thioredoxin-f-Dependent Redox Regulation" Antioxidants 10, no. 3: 401. https://doi.org/10.3390/antiox10030401

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