Impact of Silibinin A on Bioenergetics in PC12APPsw Cells and Mitochondrial Membrane Properties in Murine Brain Mitochondria
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Animals
2.3. Cell Lines
2.4. Measurement of Mitochondrial Membrane Potential (MMP)
2.5. Measurement of ATP Concentrations
2.6. High-Resolution Respirometry
2.7. Citrate Synthase Activity
2.8. Protein Content
2.9. Aβ1-40 Concentrations
2.10. ROS Concentrations
2.11. Lactate/Pyruvate Assay
2.12. Isolation of Mouse Brain Mitochondria for Mitochondrial Membrane Interactions
2.13. Mitochondrial Swelling (MS)
2.14. Mitochondrial Membrane Fluidity (MMF)
2.15. Statistics
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cells | State 3 Respiration (CI(P)) | State 4 Respiration (CI(L)) | RCR |
---|---|---|---|
PC12neo | 1.30 ± 0.17 | 0.407 ± 0.036 | 3.11 ± 0.60 |
PC12APPsw | 1.19 ± 0.040 | 0.478 ± 0.014 | 2.52 ± 0.14 pn.s. = 0.69 |
Ctrl | SIL50 | p | |
---|---|---|---|
Aβ1-40 [pg/mgProtein] | 82.22 ± 7.98 | 99.26 ± 10.08 | 0.20, n.s. |
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Esselun, C.; Bruns, B.; Hagl, S.; Grewal, R.; Eckert, G.P. Impact of Silibinin A on Bioenergetics in PC12APPsw Cells and Mitochondrial Membrane Properties in Murine Brain Mitochondria. Antioxidants 2021, 10, 1520. https://doi.org/10.3390/antiox10101520
Esselun C, Bruns B, Hagl S, Grewal R, Eckert GP. Impact of Silibinin A on Bioenergetics in PC12APPsw Cells and Mitochondrial Membrane Properties in Murine Brain Mitochondria. Antioxidants. 2021; 10(10):1520. https://doi.org/10.3390/antiox10101520
Chicago/Turabian StyleEsselun, Carsten, Bastian Bruns, Stephanie Hagl, Rekha Grewal, and Gunter P. Eckert. 2021. "Impact of Silibinin A on Bioenergetics in PC12APPsw Cells and Mitochondrial Membrane Properties in Murine Brain Mitochondria" Antioxidants 10, no. 10: 1520. https://doi.org/10.3390/antiox10101520