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Please note that, as of 18 July 2017, Microarrays has been renamed to High-Throughput and is now published here.
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Microintaglio Printing for Soft Lithography-Based in Situ Microarrays

Green Devices Research Center, School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan
Department of Bioengineering, School of Engineering, The University of Tokyo, 2-11-16 Yayoi, Bunkyo-ku, Tokyo 113-8656, Japan
Authors to whom correspondence should be addressed.
Academic Editor: Massimo Negrini
Microarrays 2015, 4(3), 311-323;
Received: 13 May 2015 / Revised: 27 June 2015 / Accepted: 9 July 2015 / Published: 14 July 2015
(This article belongs to the Special Issue New and Old Technologies for Generation of Microarrays)
Advances in lithographic approaches to fabricating bio-microarrays have been extensively explored over the last two decades. However, the need for pattern flexibility, a high density, a high resolution, affordability and on-demand fabrication is promoting the development of unconventional routes for microarray fabrication. This review highlights the development and uses of a new molecular lithography approach, called “microintaglio printing technology”, for large-scale bio-microarray fabrication using a microreactor array (µRA)-based chip consisting of uniformly-arranged, femtoliter-size µRA molds. In this method, a single-molecule-amplified DNA microarray pattern is self-assembled onto a µRA mold and subsequently converted into a messenger RNA or protein microarray pattern by simultaneously producing and transferring (immobilizing) a messenger RNA or a protein from a µRA mold to a glass surface. Microintaglio printing allows the self-assembly and patterning of in situ-synthesized biomolecules into high-density (kilo-giga-density), ordered arrays on a chip surface with µm-order precision. This holistic aim, which is difficult to achieve using conventional printing and microarray approaches, is expected to revolutionize and reshape proteomics. This review is not written comprehensively, but rather substantively, highlighting the versatility of microintaglio printing for developing a prerequisite platform for microarray technology for the postgenomic era. View Full-Text
Keywords: microarray; intaglio printing; bioprinting; in situ synthesis; microreactor array microarray; intaglio printing; bioprinting; in situ synthesis; microreactor array
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Biyani, M.; Ichiki, T. Microintaglio Printing for Soft Lithography-Based in Situ Microarrays. Microarrays 2015, 4, 311-323.

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