Next Article in Journal
Conventional Structural Magnetic Resonance Imaging in Differentiating Chronic Disorders of Consciousness
Previous Article in Journal
Patient-Derived Stem Cell Models in SPAST HSP: Disease Modelling and Drug Discovery
Open AccessArticle

New Insights into GFAP Negative Astrocytes in Calbindin D28k Immunoreactive Astrocytes

by Jie Xu 1,2
1
Department of Cellular Physiology (in Biomedical Centre Munich—BMC), Ludwig-Maximilians-University Munich, 82152 Planegg-Martinsried, Germany
2
Wicker Clinic, 61348 Bad Homburg, Germany
Brain Sci. 2018, 8(8), 143; https://doi.org/10.3390/brainsci8080143
Received: 26 May 2018 / Revised: 27 July 2018 / Accepted: 31 July 2018 / Published: 3 August 2018
Glial fibrillary acidic protein (GFAP) is commonly used as a specific marker for the identification of astrocytes. Nevertheless, it is known from the literature that astrocytes in situ in contrast to cultured astrocytes may feature lower levels of GFAP. In order to characterize the properties of GFAP in Calbindin D28k immunoreactive astrocytes, we use primary astrocyte cultures from cells of new-born mice. A double fluorescence immunocytochemical analysis reveals that GFAP in cultured Calbindin D28k astrocytes behaves differently depending on whether the medium contains foetal bovine serum (FBS) or not. The novelty in our study is, however, that a high percentage of Calbindin D28k cultured astrocytes in a medium with 10% FBS are GFAP negative. In addition, the study shows that Calbindin D28k astrocytes have (i) a different morphology and (ii) a higher concentration of Calbindin D28k in the nucleus than in the cytoplasm. The study provides new evidence that in order to fully understand the characteristics of astrocytes, astrocytes which are Calbindin D28k positive have to be investigated. View Full-Text
Keywords: glial fibrillary acidic protein (GFAP); Calbindin D28k; cultured astrocytes glial fibrillary acidic protein (GFAP); Calbindin D28k; cultured astrocytes
Show Figures

Figure 1

MDPI and ACS Style

Xu, J. New Insights into GFAP Negative Astrocytes in Calbindin D28k Immunoreactive Astrocytes. Brain Sci. 2018, 8, 143.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop