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Open AccessArticle

Preliminary Investigation of Species Diversity of Rice Hopper Parasitoids in Southeast Asia

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Agricultural Entomology, Department of Crop Sciences, University of Göttingen, Grisebachstr. 6, 37077 Göttingen, Germany
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Applied Marine and Estuarine Ecology, School of Biological, Earth and Environmental Sciences, The University of New South Wales (UNSW), Sydney, NSW 2052, Australia
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Molecular Ecology, Institute of Biology, Martin-Luther-University Halle-Wittenberg, Hoher Weg 4, 06099 Halle (Saale), Germany
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Abeilles et Environment, INRA PACA, 84914 Avignon CEDEX 9, France
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Department of Genomic and Applied Microbiology, Institute of Microbiology and Genetics, University of Göttingen, Grisebachstr. 8, 37077 Göttingen, Germany
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Authors to whom correspondence should be addressed.
Insects 2018, 9(1), 19; https://doi.org/10.3390/insects9010019
Received: 13 November 2017 / Revised: 5 January 2018 / Accepted: 26 January 2018 / Published: 9 February 2018
Ongoing intensification of rice production systems in Southeast Asia is causing devastating yield losses each year due to rice hoppers. Their continuing development of immunity to resistant rice varieties and pesticide applications further complicates this problem. Hence, there is a high demand for biological control agents of rice hoppers. Egg parasitoid wasps are among the most important natural enemies of rice hoppers, such as Nilaparvata lugens and Nephotettix spp. However, our knowledge of their diversity is still very limited, due to their small size and the lack of available morphological information. Classifying these parasitoids is the first step to properly understanding their role in the rice agroecosystem. We used traditional morphological identification, as well as DNA sequencing of the 28S rRNA and the COI genes, to investigate the diversity of four important hopper egg parasitoid genera in the Philippines. Parasitoids of the genera Anagrus, Oligosita, Gonatocerus, and Paracentrobia were collected in eight study landscapes located in Luzon. Our findings illustrate that characterization of species diversity using morphological and molecular analyses were concordant only for the genus Paracentrobia. The genera Anagrus and Gonatocerus exhibited more genetic diversity than estimated with the morphological analysis, while the opposite was observed for Oligosita. This is the first study investigating the molecular diversity of rice hopper parasitoids in the Philippines. More research combining morphological, behavioral, and molecular methods, as well as the establishment of a comprehensive DNA database, are urgently needed to assess the performance and suitability of these organisms as biocontrol agents. View Full-Text
Keywords: DNA barcoding; genetic diversity; hymenopteran parasitoids; Nephotettix; Nilaparvata lugens; rice DNA barcoding; genetic diversity; hymenopteran parasitoids; Nephotettix; Nilaparvata lugens; rice
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Sann, C.; Wemheuer, F.; Beaurepaire, A.; Daniel, R.; Erler, S.; Vidal, S. Preliminary Investigation of Species Diversity of Rice Hopper Parasitoids in Southeast Asia. Insects 2018, 9, 19.

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