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Article

Differential Proteomics Analysis Unraveled Mechanisms of Arma chinensis Responding to Improved Artificial Diet

1
Mass Production Base of Natural Enemy Insects of Tianjin Academy of Agricultural Sciences, Institute of Plant Protection, Tianjin Academy of Agricultural Sciences, Tianjin 300384, China
2
Biological Control of Insects Research Laboratory, USDA-Agricultural Research Service, Columbia, MO 65203-3535, USA
3
College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300392, China
4
Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
*
Author to whom correspondence should be addressed.
Academic Editor: Allen Carson Cohen
Insects 2022, 13(7), 605; https://doi.org/10.3390/insects13070605
Received: 25 May 2022 / Revised: 25 June 2022 / Accepted: 28 June 2022 / Published: 2 July 2022
(This article belongs to the Special Issue Advances in Insect Diet and Rearing Methodology (Volume II))
Arma chinensis Fallou is a predaceous stink bug that can effectively control many kinds of agricultural and forest pests, such as fall armyworm, cotton bollworm and Colorado potato beetle. An insect-free artificial diet comprising chicken egg, tuna fish and raw pig liver was developed for A. chinensis. Several biological characteristics were diminished for A. chinensis reared on the artificial diet compared to the pupae of Chinese oak silk moth. Changes in the formulation of the diet were made in response to the transcriptome results and tested using biological characteristics. Several parameters were improved over 6 generations, although the improved artificial diet remained inferior to the pupae of Chinese oak silk moth regarding egg viability, fecundity, body weight, and nymphal development time. The current study reported the differential proteomic analysis revealing the mechanism of A. chinensis responding to the improved artificial diet. This information will be used to optimize the formulation of the artificial diet and decrease the cost of mass rearing in A. chinensis.
The development of artificial diets could considerably simplify and reduce the cost of mass rearing of natural enemies compared to conventional rearing methods. However, improvement of artificial diets can be tedious, convoluted and often uncertain. For accelerating diet development, a better method that can offer informative feedback to target deficiencies in diet improvement is required. Our previous research demonstrated several biological characteristics were diminished in the insect predator, Arma chinensis Fallou, fed on an artificial diet formulated with the aid of transcriptomic methods compared to the Chinese oak silk moth pupae. The present study reports differential proteomic analysis by iTRAQ-PRM, which unravels the molecular mechanism of A. chinensis responding to improvements in the artificial diet. Our study provides multivariate proteomic data and provides comprehensive sequence information in studying A. chinensis. Further, the physiological roles of the differentially expressed proteins and pathways enable us to explain several biological differences between natural prey-fed and improved diet-fed A. chinensis, and subsequent proposed reformulation optimizations to artificial diets. View Full-Text
Keywords: Arma chinensis; mass rearing; artificial diet; reformulation; nutriproteomics; biological control Arma chinensis; mass rearing; artificial diet; reformulation; nutriproteomics; biological control
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MDPI and ACS Style

Zou, D.; Coudron, T.A.; Wu, H.; Zhang, L.; Wang, M.; Xu, W.; Xu, J.; Song, L.; Xiao, X. Differential Proteomics Analysis Unraveled Mechanisms of Arma chinensis Responding to Improved Artificial Diet. Insects 2022, 13, 605. https://doi.org/10.3390/insects13070605

AMA Style

Zou D, Coudron TA, Wu H, Zhang L, Wang M, Xu W, Xu J, Song L, Xiao X. Differential Proteomics Analysis Unraveled Mechanisms of Arma chinensis Responding to Improved Artificial Diet. Insects. 2022; 13(7):605. https://doi.org/10.3390/insects13070605

Chicago/Turabian Style

Zou, Deyu, Thomas A. Coudron, Huihui Wu, Lisheng Zhang, Mengqing Wang, Weihong Xu, Jingyang Xu, Liuxiao Song, and Xuezhuang Xiao. 2022. "Differential Proteomics Analysis Unraveled Mechanisms of Arma chinensis Responding to Improved Artificial Diet" Insects 13, no. 7: 605. https://doi.org/10.3390/insects13070605

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