Are Semi-Quantitative Clinical Cultures Inadequate? Comparison to Quantitative Analysis of 1053 Bacterial Isolates from 350 Wounds
Abstract
:1. Introduction
2. Materials and Methods
2.1. Wound Biopsy, Semi-Quantitative and Quantitative Culture Analysis
2.2. Fluorescence Imaging Procedure
3. Results
4. Discussion
Proposed Solution
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Semi-Quantitative Culture Categories | Quantitative Culture Values | |
---|---|---|
Mean (SE) | Median (Range) | |
Occasional | 4.9 × 104 CFU/g (3.1 × 104 CFU/g) | 6.6 × 103 CFU/g (3.1 × 102 CFU/g–7.3 × 106 CFU/g) |
Light | 2.5 × 105 CFU/g (6.3 × 104 CFU/g) | 8.6 × 104 CFU/g (3.0 × 103–1.4 × 107 CFU/g) |
Moderate | 5.4 × 106 CFU/g (6.1 × 105 CFU/g) | 1.6 × 106 CFU/g (1.6 × 104–7.3 × 107 CFU/g) |
Heavy | 1.4 × 108 CFU/g (2.9 × 107 CFU/g) | 3.5 × 107 CFU/g (2.9 × 104–7.0 × 109 CFU/g) |
Semi-Quantitative Category | ||||
---|---|---|---|---|
Quantitative Bacterial Load | Occasional N = 238 | Light N = 246 | Moderate N = 272 | Heavy N = 257 |
<104 CFU/g | 142 | 10 | 0 | 0 |
104 CFU/g | 88 | 127 | 8 | 1 |
105 CFU/g | 7 | 103 | 99 | 7 |
106 CFU/g | 1 | 5 | 128 | 64 |
107 CFU/g | 0 | 1 | 37 | 97 |
>108 CFU/g | 0 | 0 | 0 | 88 |
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Serena, T.E.; Bowler, P.G.; Schultz, G.S.; D’souza, A.; Rennie, M.Y. Are Semi-Quantitative Clinical Cultures Inadequate? Comparison to Quantitative Analysis of 1053 Bacterial Isolates from 350 Wounds. Diagnostics 2021, 11, 1239. https://doi.org/10.3390/diagnostics11071239
Serena TE, Bowler PG, Schultz GS, D’souza A, Rennie MY. Are Semi-Quantitative Clinical Cultures Inadequate? Comparison to Quantitative Analysis of 1053 Bacterial Isolates from 350 Wounds. Diagnostics. 2021; 11(7):1239. https://doi.org/10.3390/diagnostics11071239
Chicago/Turabian StyleSerena, Thomas E., Philip G. Bowler, Gregory S. Schultz, Anna D’souza, and Monique Y. Rennie. 2021. "Are Semi-Quantitative Clinical Cultures Inadequate? Comparison to Quantitative Analysis of 1053 Bacterial Isolates from 350 Wounds" Diagnostics 11, no. 7: 1239. https://doi.org/10.3390/diagnostics11071239