Lack of Association between Chlamydophila psittaci and Ocular Adnexal MALT Lymphoma in Korean Patients—Is the Geographic or Genetic Difference Significant?
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patient and Tissue Samples
2.2. Molecular Detection of C. psittaci
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
- Moslehi, R.; Devesa, S.S.; Schairer, C.; Fraumeni, J.F., Jr. Rapidly increasing incidence of ocular non-Hodgkin lymphoma. J. Natl. Cancer Inst. 2006, 98, 936–939. [Google Scholar] [CrossRef] [PubMed]
- Ferreri, A.J.; Guidoboni, M.; Ponzoni, M.; De Conciliis, C.; Dell’Oro, S.; Fleischhauer, K.; Caggiari, L.; Lettini, A.A.; Dal Cin, E.; Ieri, R.; et al. Evidence for an association between Chlamydia psittaci and ocular adnexal lymphomas. J. Natl. Cancer Inst. 2004, 96, 586–594. [Google Scholar] [CrossRef] [PubMed]
- Yoo, C.; Ryu, M.H.; Huh, J.; Park, J.H.; Kang, H.J.; Ahn, H.S.; Lee, Y.; Kim, M.J.; Lee, H.; Kim, T.W.; et al. Chlamydia psittaci infection and clinicopathologic analysis of ocular adnexal lymphomas in Korea. Am. J. Hematol. 2007, 82, 821–823. [Google Scholar] [CrossRef] [PubMed]
- Decaudin, D.; Dolcetti, R.; de Cremoux, P.; Ponzoni, M.; Vincent-Salomon, A.; Doglioni, C.; Dendale, R.; Escande, M.C.; Lumbroso-Le Rouic, L.; Ferreri, A.J. Variable association between Chlamydophila psittaci infection and ocular adnexal lymphomas: Methodological biases or true geographical variations? Anti-Cancer Drugs 2008, 19, 761–765. [Google Scholar] [CrossRef] [PubMed]
- Vargas, R.L.; Fallone, E.; Felgar, R.E.; Friedberg, J.W.; Arbini, A.A.; Andersen, A.A.; Rothberg, P.G. Is there an association between ocular adnexal lymphoma and infection with Chlamydia psittaci? The University of Rochester experience. Leuk. Res. 2006, 30, 547–551. [Google Scholar] [CrossRef] [PubMed]
- Daibata, M.; Nemoto, Y.; Togitani, K.; Fukushima, A.; Ueno, H.; Ouchi, K.; Fukushi, H.; Imai, S.; Taguchi, H. Absence of Chlamydia psittaci in ocular adnexal lymphoma from Japanese patients. Br. J. Haematol. 2006, 132, 651–652. [Google Scholar] [CrossRef] [PubMed]
- Madico, G.; Quinn, T.C.; Boman, J.; Gaydos, C.A. Touchdown enzyme time release-PCR for detection and identification of Chlamydia trachomatis, C. pneumoniae, and C. psittaci using the 16S and 16S-23S spacer rRNA genes. J. Clin. Microbiol. 2000, 38, 1085–1093. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ferreri, A.J.; Govi, S.; Ponzoni, M. Marginal zone lymphomas and infectious agents. Semin. Cancer Biol. 2013, 23, 431–440. [Google Scholar] [CrossRef] [PubMed]
- Tovilla-Canales, J.L.; Pomar, Y.J.L.T.; Ceron, J.R.; Pomar, Y.J.L.T.; Ceron, J.R. Lymphoproliferative disorders of the ocular adnexa. Curr. Opin. Ophthalmol. 2004, 15, 401–405. [Google Scholar] [CrossRef] [PubMed]
- Ferreri, A.J.; Ponzoni, M.; Guidoboni, M.; Resti, A.G.; Politi, L.S.; Cortelazzo, S.; Demeter, J.; Zallio, F.; Palmas, A.; Muti, G.; et al. Bacteria-eradicating therapy with doxycycline in ocular adnexal MALT lymphoma: A multicenter prospective trial. J. Natl. Cancer Inst. 2006, 98, 1375–1382. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ferreri, A.J.; Govi, S.; Pasini, E.; Mappa, S.; Bertoni, F.; Zaja, F.; Montalban, C.; Stelitano, C.; Cabrera, M.E.; Giordano Resti, A.; et al. Chlamydophila psittaci eradication with doxycycline as first-line targeted therapy for ocular adnexae lymphoma: Final results of an international phase II trial. J. Clin. Oncol. Off. J. Am. Soc. Clin. Oncol. 2012, 30, 2988–2994. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ferreri, A.J.; Dolcetti, R.; Dognini, G.P.; Malabarba, L.; Vicari, N.; Pasini, E.; Ponzoni, M.; Cangi, M.G.; Pecciarini, L.; Resti, A.G.; et al. Chlamydophila psittaci is viable and infectious in the conjunctiva and peripheral blood of patients with ocular adnexal lymphoma: Results of a single-center prospective case-control study. Int. J. Cancer 2008, 123, 1089–1093. [Google Scholar] [CrossRef] [PubMed]
- Chanudet, E.; Zhou, Y.; Bacon, C.M.; Wotherspoon, A.C.; Muller-Hermelink, H.K.; Adam, P.; Dong, H.Y.; de Jong, D.; Li, Y.; Wei, R.; et al. Chlamydia psittaci is variably associated with ocular adnexal MALT lymphoma in different geographical regions. J. Pathol. 2006, 209, 344–351. [Google Scholar] [CrossRef] [PubMed]
- Johnson, J.G.; Terpak, L.A.; Margo, C.E.; Setoodeh, R. Extranodal Marginal Zone B-cell Lymphoma of the Ocular Adnexa. Cancer Control. 2016, 23, 140–149. [Google Scholar] [CrossRef] [PubMed]
- Carugi, A.; Onnis, A.; Antonicelli, G.; Rossi, B.; Mannucci, S.; Luzzi, A.; Lazzi, S.; Bellan, C.; Tosi, G.M.; Sayed, S.; et al. Geographic variation and environmental conditions as cofactors in Chlamydia psittaci association with ocular adnexal lymphomas: A comparison between Italian and African samples. Hematol. Oncol. 2010, 28, 20–26. [Google Scholar] [CrossRef] [PubMed]
- Lee, M.J.; Min, B.J.; Choung, H.K.; Kim, N.; Kim, Y.A.; Khwarg, S.I. Genome-wide DNA methylation profiles according to Chlamydophila psittaci infection and the response to doxycycline treatment in ocular adnexal lymphoma. Mol. Vis. 2014, 20, 1037–1047. [Google Scholar] [PubMed]
- Choung, H.K.; Kim, Y.A.; Lee, M.J.; Kim, N.; Khwarg, S.I. Multigene methylation analysis of ocular adnexal MALT lymphoma and their relationship to Chlamydophila psittaci infection and clinical characteristics in South Korea. Investig. Ophthalmol. Vis. Sci. 2012, 53, 1928–1935. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ejima, Y.; Sasaki, R.; Okamoto, Y.; Maruta, T.; Azumi, A.; Hayashi, Y.; Demizu, Y.; Ota, Y.; Soejima, T.; Sugimura, K. Ocular adnexal mucosa-associated lymphoid tissue lymphoma treated with radiotherapy. Radiother. Oncol. J. Eur. Soc. Ther. Radiol. Oncol. 2006, 78, 6–9. [Google Scholar] [CrossRef] [PubMed]
Primer No. | Title 2 | Amplicon Size (bp) | Reference |
---|---|---|---|
Primer 1 (CPS 100/101) | Forward: 5′-CCC AAG GTG AGG CTG ATG AC-3′ Reverse: 5′-CAA ACC GTC CTA AGA CAG TTA-3′ | 111 | Madico et al. [7] |
Primer 2 | Forward: 5′-ATA CAG GGT GAT AGT CCC GTA GAC-3′ Reverse: 5′-GTG GTC TCC CCA GAT TCA GAC TAG-3′ | 100 | Ferreri et al. [2] |
Primer 3 (1st step primer for nested PCR) | Forward: 5′-ACG GAA TAA TGA CTT CGG-3′ Reverse: 5′-TAC CTG GTA CGC TCA ATT-3′ | 430 | Vargas et al. [5] |
Primer 4 (2nd step primer for nested PCR) | Forward: 5′-ATA ATG ACT TCG GTT CTT ATT-3′ Reverse: 5′-TGT TTT AGA TGC CTA AAC AT-3′ | 127 | Daibata et al. [6] |
Primer 5 (Newly designed primers) | Forward: 5′-CGT TGA CTC AAC CTG CAA AG-3′ Reverse: 5′-CAA CCT AGT CAA ACC GTC CT-3′ | 149 | - |
Characteristics | Number of Patients (n = 150) | Percentage |
---|---|---|
Age, years | ||
Median (range) | 39 (24–63) | |
Sex | ||
Female | 107 | 71% |
Male | 43 | 29% |
Site of tumor | ||
Conjunctiva | 125 | 83% |
Lacrimal gland | 0 | 0% |
Eyelids | 6 | 4% |
Lacrimal drainage apparatus | 12 | 8% |
Other orbital tissues | 7 | 5% |
Laterality | ||
Unilateral | 104 | 69% |
Bilateral | 46 | 31% |
Residency in rural areas | 23 | 15% |
TNM stage | ||
Stage I | 141 | 94% |
Stage II-III | 9 | 6% |
Treatment | ||
RT | 118 | 79% |
CVP | 15 | 10% |
RT + CVP | 7 | 5% |
No additional treatment | 10 | 7% |
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Cho, U.; Cho, I.; Lee, S.H.; Yang, S.W.; Cho, S.G.; Lee, Y.S.; Lee, H.W.; Park, G. Lack of Association between Chlamydophila psittaci and Ocular Adnexal MALT Lymphoma in Korean Patients—Is the Geographic or Genetic Difference Significant? Diagnostics 2021, 11, 2069. https://doi.org/10.3390/diagnostics11112069
Cho U, Cho I, Lee SH, Yang SW, Cho SG, Lee YS, Lee HW, Park G. Lack of Association between Chlamydophila psittaci and Ocular Adnexal MALT Lymphoma in Korean Patients—Is the Geographic or Genetic Difference Significant? Diagnostics. 2021; 11(11):2069. https://doi.org/10.3390/diagnostics11112069
Chicago/Turabian StyleCho, Uiju, Inju Cho, Sung Hak Lee, Suk Woo Yang, Seok Goo Cho, Youn Soo Lee, Hye Won Lee, and Gyeongsin Park. 2021. "Lack of Association between Chlamydophila psittaci and Ocular Adnexal MALT Lymphoma in Korean Patients—Is the Geographic or Genetic Difference Significant?" Diagnostics 11, no. 11: 2069. https://doi.org/10.3390/diagnostics11112069