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Review
Peer-Review Record

The Fate and Occurrence of Antibiotic-Resistant Bacteria and Antibiotic Resistance Genes during Advanced Wastewater Treatment and Disinfection: A Review

Water 2023, 15(11), 2084; https://doi.org/10.3390/w15112084
by Maria Kalli, Constantinos Noutsopoulos and Daniel Mamais *
Reviewer 1:
Reviewer 2:
Reviewer 3: Anonymous
Water 2023, 15(11), 2084; https://doi.org/10.3390/w15112084
Submission received: 2 May 2023 / Revised: 28 May 2023 / Accepted: 29 May 2023 / Published: 31 May 2023

Round 1

Reviewer 1 Report

Very complete review on the topic; the authors provide a state-of-the-art insight into what is already known, and what is still unmet or missing.

I would suggest shortening the Introduction, as it already jumps into many points that have to be treated and discussed later on in the manuscript.

Very well-written, with clear tables.

Author Response

Please see the attachment. 

Author Response File: Author Response.docx

Reviewer 2 Report

The review is interesting, useful and well prepared.

Introduction it is in line with the Instructions for the Authors. In this review, the authors evaluated the potential removal of ARB and ARGs by disinfection methods, advanced oxidation processes, and tertiary wastewater treatment. Conventional wastewater treatment plants are not capable of efficiently removing ARB and ARGs, and therefore their coupling with advanced wastewater treatment processes is essential to  hinder the spread of antibiotic resistance in water bodies.

The methodology is also corresponding to the review  points of interest.

Discussion is also appropriate, but a more precise direction of future research should add more value.

References are in line with the scientific demonstration of the main issues.

 

Author Response

Please see the attachment. 

 

Author Response File: Author Response.docx

Reviewer 3 Report

The review focuses on a global problem that is currently on the agenda of the UN, and it is commendable that efforts are being made in this direction (source: https://www.un.org/sustainabledevelopment/water-and-sanitation/). The authors discuss various methods of water purification from bacteria, such as membrane filtration, adsorption, chemical treatment, catalysis, and photocatalysis. However, they have not taken into account a class of advanced antibacterial agents that are commonly used today, primarily copper-based agents (source: https://doi.org/10.1016/j.enceco.2020.12.001, doi: 10.2174/1381612824666180219130659, https://doi.org/10.1021/acsomega.0c01335, https://doi.org/10.1021/acsabm.9b00754, https://doi.org/10.3390/catal13020348). These promising antimicrobial agents are environmentally friendly substances that do not require additional introduction of oxidants, and so on.

 

Overall, the review is well-written, and the information is logically presented. However, it would be beneficial for the authors to address or provide an explanation regarding the expected effectiveness of these water purifiers, based on the mechanism of bacterial death.

Author Response

Please see the attachment. 

 

Author Response File: Author Response.docx

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