The present study aimed to identify the seasonal changes in the hydrochemical characteristics of the Huixian karst wetland and the underlying mechanism. Conventional ions and isotopes of 130 groundwater samples collected during the wet and dry seasons were analyzed. The hydrochemical characteristics of groundwater in the Huixian karst wetland were clarified using mathematical statistics and hydrochemical methods, including Durov diagrams, ionic ratios, Gibbs diagrams, and H and O isotopes. The hydrochemical evolution and sources of major ions in the Huixian karst wetland were also investigated. The results showed that cations and anions in groundwater in the study area were dominated by Ca2+
, respectively, sourced mainly from calcite weathering. The ions of some groundwater samples were regulated by weathering of dolomite, dolomitic limestone, and pyrite, resulting in relatively high concentrations of Mg2+
, and Cl−
in groundwater originated from precipitation, Na+
originated from domestic sewage, K+
was related to the application of potassium fertilizer in agriculture, and NO3−
mainly originated from chemical fertilizer. Groundwater ions were primarily controlled by rock weathering, followed by evaporative concentration. The sources of major ions were dependent on the dissolution and precipitation of carbonate rock, as well as the chemical weathering of silicate rock and evaporite. Samples from the various water sources were scattered on both sides of the local meteoric water line (δD = 3.13δ18
O − 13.9), which indicated isotopic composition was affected by evaporation and precipitation.
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