Denitrification-Potential Evaluation and Nitrate-Removal-Pathway Analysis of Aerobic Denitrifier Strain Marinobacter hydrocarbonoclasticus RAD-2
Abstract
:1. Introduction
2. Materials and Methods
2.1. Culture Media
2.2. Bacteria Isolation, Screening, and Identification
2.3. Nitrogen-Removal Performance Evaluation
2.4. RT-qPCR Analysis
2.5. Analytical Methods
3. Results
3.1. Characteristics and Identification
3.2. Nitrogen Removal Performance Evaluation
3.2.1. Aerobic Nitrogen-Removal Ability of Strain RAD-2
3.2.2. Single-Factor Experiments of Strain RAD-2
3.3. Expression of Denitrifying Genes by RT-qPCR Analysis
4. Discussion
4.1. Characteristics and Identification
4.2. Nitrogen-Removal Performance Evaluation
4.3. Aerobic Denitrification Pathways Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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API 20 NE Results | Strain RAD-2 |
---|---|
Oxidase test | + |
Nitrate reduction | + |
Arginine dihydrolase | - |
Urease | - |
β-glucosidase | - |
Protease | - |
β-galactosidase | - |
Assimilation of Glucose | + |
Arabinose | - |
Mannose | - |
Mannitol | - |
N-acetyl-glucosamine | - |
Maltose | - |
Gluconate | + |
Capric acid | - |
Adipic acid | - |
Malic acid | - |
Citric acid | - |
Phenylacetic acid | - |
Factor | Variations | Growth (OD600) | Initial Nitrate Concentration (mg/L) | Final Nitrate Concentration (mg/L) | Removal Efficiency (%) |
---|---|---|---|---|---|
C/N Ratio | 2 | 0.32 ± 0.03 | 306.33 ± 0.95 | 203.44 ± 13.05 | 33.59 ± 4.17 |
5 | 0.79 ± 0.13 | 305.25 ± 0.65 | 4.06 ± 0.58 | 98.67 ± 0.19 | |
10 | 1.34 ± 0.05 | 305.21 ± 0.31 | 6.48 ± 1.93 | 97.88 ± 0.63 | |
15 | 0.94 ± 0.18 | 304.02 ± 0.26 | 40.72 ± 5.77 | 86.61 ± 1.90 | |
20 | 0.42 ± 0.05 | 304.67 ± 0.45 | 213.90 ± 12.26 | 29.80 ± 3.92 | |
Temperature (°C) | 5 | 0.012 ± 0.06 | 306.37 ± 0.36 | 305.74 ± 0.40 | 0.21 ± 0.25 |
10 | 0.11 ± 0.03 | 305.38 ± 0.35 | 298.75 ± 2.59 | 2.17 ± 0.85 | |
15 | 0.24 ± 0.06 | 304.01 ± 0.38 | 274.22 ± 6.48 | 9.79 ± 2.25 | |
25 | 1.12 ± 0.12 | 303.29 ± 0.17 | 6.23 ± 3.61 | 97.95 ± 1.19 | |
40 | 1.02 ± 0.19 | 304.52 ± 0.37 | 10.18 ± 1.18 | 96.66 ± 0.39 | |
Rotation Speed (rpm) | 0 | 0.52 ± 0.11 | 304.86 ± 0.50 | 138.99 ± 17.06 | 54.41 ± 5.53 |
50 | 0.67 ± 0.05 | 303.62 ± 0.30 | 92.14 ± 11.05 | 69.65 ± 3.61 | |
100 | 0.95 ± 0.13 | 305.34 ± 0.21 | 24.38 ± 6.33 | 92.02 ± 2.08 | |
150 | 1.25 ± 0.11 | 305.38 ± 0.24 | 7.02 ± 1.51 | 97.70 ± 0.49 | |
200 | 1.03 ± 0.07 | 304.43 ± 0.27 | 30.45 ± 8.37 | 89.90 ± 2.75 |
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Kong, D.; Li, W.; Deng, Y.; Ruan, Y.; Chen, G.; Yu, J.; Lin, F. Denitrification-Potential Evaluation and Nitrate-Removal-Pathway Analysis of Aerobic Denitrifier Strain Marinobacter hydrocarbonoclasticus RAD-2. Water 2018, 10, 1298. https://doi.org/10.3390/w10101298
Kong D, Li W, Deng Y, Ruan Y, Chen G, Yu J, Lin F. Denitrification-Potential Evaluation and Nitrate-Removal-Pathway Analysis of Aerobic Denitrifier Strain Marinobacter hydrocarbonoclasticus RAD-2. Water. 2018; 10(10):1298. https://doi.org/10.3390/w10101298
Chicago/Turabian StyleKong, Dedong, Wenbing Li, Yale Deng, Yunjie Ruan, Guangsuo Chen, Jianhai Yu, and Fucheng Lin. 2018. "Denitrification-Potential Evaluation and Nitrate-Removal-Pathway Analysis of Aerobic Denitrifier Strain Marinobacter hydrocarbonoclasticus RAD-2" Water 10, no. 10: 1298. https://doi.org/10.3390/w10101298