Next Article in Journal
Evolutionary Divergent Suppressor Mutations in Conformational Diseases
Next Article in Special Issue
Bioengineering Strategies for Protein-Based Nanoparticles
Previous Article in Journal
Ensemble Consensus-Guided Unsupervised Feature Selection to Identify Huntington’s Disease-Associated Genes
Previous Article in Special Issue
The Multiplanetary Future of Plant Synthetic Biology
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessReview
Genes 2018, 9(7), 351;

Targeted Approaches for In Situ Gut Microbiome Manipulation

Department of Biochemistry, Yong Loo Lin School of Medicine, 8 Medical Drive, Singapore 117596, Singapore
NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, 28 Medical Drive, Singapore 117456, Singapore
Department of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Rd, Singapore 119074, Singapore
Khoo Teck Puat-National University Children’s Medical Institute, National University Health System, 5 Lower Kent Ridge Rd, Singapore 119074, Singapore
Contributed equally.
Author to whom correspondence should be addressed.
Received: 28 May 2018 / Revised: 7 July 2018 / Accepted: 9 July 2018 / Published: 12 July 2018
(This article belongs to the Special Issue Emerging Applications in Synthetic Biology)
Full-Text   |   PDF [1002 KB, uploaded 12 July 2018]   |  


Microbial communities and their collective genomes form the gut microbiome, of which bacteria are the major contributor. Through their secreted metabolites, bacteria interact with the host, influencing human health and physiology. Perturbation of the microbiota and metabolome has been associated with various diseases and metabolic conditions. As knowledge on fundamental host-microbiome interactions and genetic engineering tools becomes readily available, targeted manipulation of the gut microbiome for therapeutic applications gains favourable attention. Manipulation of the gut microbiome can be achieved by altering the microbiota population and composition, or by modifying the functional metabolic activity of the microbiome to promote health and restore the microbiome balance. In this article, we review current works that demonstrate various strategies employed to manipulate the gut microbiome in situ to various degrees of precision. View Full-Text
Keywords: gut microbiome; microbiome modulation; prebiotics; probiotics; phage; CRISPR-Cas9 gut microbiome; microbiome modulation; prebiotics; probiotics; phage; CRISPR-Cas9

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Lee, H.L.; Shen, H.; Hwang, I.Y.; Ling, H.; Yew, W.S.; Lee, Y.S.; Chang, M.W. Targeted Approaches for In Situ Gut Microbiome Manipulation. Genes 2018, 9, 351.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top