Genes 2017, 8(11), 330; https://doi.org/10.3390/genes8110330
The HMGA1 Pseudogene 7 Induces miR-483 and miR-675 Upregulation by Activating Egr1 through a ceRNA Mechanism
Marco De Martino 1, Giuseppe Palma 2
, Amalia Azzariti 3, Claudio Arra 2, Alfredo Fusco 1 and Francesco Esposito 1,*
, Amalia Azzariti 3, Claudio Arra 2, Alfredo Fusco 1 and Francesco Esposito 1,*
1
Istituto di Endocrinologia ed Oncologia Sperimentale del CNR c/o Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Scuola di Medicina e Chirurgia di Napoli, Università degli Studi di Napoli “Federico II”, via Pansini, 5, 80131 Naples, Italy
2
Istituto Nazionale dei Tumori, Fondazione Pascale, via Mariano Semmola, 52, 80131 Naples, Italy
3
IRCCS Istituto Tumori Giovanni Paolo II, Viale O. Flacco, 65, 70124 Bari, Italy
*
Author to whom correspondence should be addressed.
Received: 31 October 2017 / Revised: 8 November 2017 / Accepted: 9 November 2017 / Published: 17 November 2017
(This article belongs to the Special Issue Non-coding RNAs)
Abstract
Several studies have established that pseudogene mRNAs can work as competing endogenous RNAs and, when deregulated, play a key role in the onset of human neoplasias. Recently, we have isolated two HMGA1 pseudogenes, HMGA1P6 and HMGA1P7. These pseudogenes have a critical role in cancer progression, acting as micro RNA (miRNA) sponges for HMGA1 and other cancer-related genes. HMGA1 pseudogenes were found overexpressed in several human carcinomas, and their expression levels positively correlate with an advanced cancer stage and a poor prognosis. In order to investigate the molecular alterations following HMGA1 pseudogene 7 overexpression, we carried out miRNA sequencing analysis on HMGA1P7 overexpressing mouse embryonic fibroblasts. Intriguingly, the most upregulated miRNAs were miR-483 and miR-675 that have been described as key regulators in cancer progression. Here, we report that HMGA1P7 upregulates miR-483 and miR-675 through a competing endogenous RNA mechanism with Egr1, a transcriptional factor that positively regulates miR-483 and miR-675 expression. View Full-TextKeywords:
HMGA1 pseudogenes; HMGA1P7; micro RNA; ceRNA
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De Martino, M.; Palma, G.; Azzariti, A.; Arra, C.; Fusco, A.; Esposito, F. The HMGA1 Pseudogene 7 Induces miR-483 and miR-675 Upregulation by Activating Egr1 through a ceRNA Mechanism. Genes 2017, 8, 330.
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