Silencing of GhSINAT5 Reduces Drought Resistance and Salt Tolerance in Cotton
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Extraction of Total Cotton RNA and Synthesis of the First Strand of cDNA
2.3. Quantitative Real-Time Polymerase Chain Reaction
2.4. Gene Cloning and Bioinformatics Analysis
2.5. VIGS and Gene Expression Level Detection
2.6. Cotton Stress Treatment
2.7. Determination of Physiological and Biochemical Indicators
2.8. Statistical Data
3. Results
3.1. Cloning and Sequence Analysis of GhSINAT5
3.2. GhSINAT5 Expression Pattern Analysis
3.3. Silencing GhSINAT5 Reduces Drought Resistance in Cotton
3.4. Silencing of GhSINAT5 Increases the Extent of Membrane Injury and Decreases Antioxidant Enzyme Activity in Cotton Cells
3.5. GhSINAT5 Regulates the Expression of Key Genes Involved in Drought Stress
3.6. Silencing of GhSINAT5 Reduces Salt Resistance in Cotton
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Wang, Y.; Zeng, J.; Yu, Y.; Ni, Z. Silencing of GhSINAT5 Reduces Drought Resistance and Salt Tolerance in Cotton. Genes 2024, 15, 1063. https://doi.org/10.3390/genes15081063
Wang Y, Zeng J, Yu Y, Ni Z. Silencing of GhSINAT5 Reduces Drought Resistance and Salt Tolerance in Cotton. Genes. 2024; 15(8):1063. https://doi.org/10.3390/genes15081063
Chicago/Turabian StyleWang, Yi, Jiacong Zeng, Yuehua Yu, and Zhiyong Ni. 2024. "Silencing of GhSINAT5 Reduces Drought Resistance and Salt Tolerance in Cotton" Genes 15, no. 8: 1063. https://doi.org/10.3390/genes15081063
APA StyleWang, Y., Zeng, J., Yu, Y., & Ni, Z. (2024). Silencing of GhSINAT5 Reduces Drought Resistance and Salt Tolerance in Cotton. Genes, 15(8), 1063. https://doi.org/10.3390/genes15081063