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Article

Impact of Group II Baculovirus IAPs on Virus-Induced Apoptosis in Insect Cells

Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding & Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Pedro Lorite Martínez
Genes 2022, 13(5), 750; https://doi.org/10.3390/genes13050750
Received: 18 March 2022 / Revised: 19 April 2022 / Accepted: 22 April 2022 / Published: 24 April 2022
(This article belongs to the Section Animal Genetics and Genomics)
Apoptosis plays an important role in virus-host interactions and is a major element of the insect immune response. Exploring the regulatory mechanisms of virus-induced apoptosis through the expression of apoptotic genes holds important research and application value. Functional research on the reported inhibitor of apoptosis proteins (IAPs) mainly focuses on the group I baculovirus, while the functions of the group II baculovirus IAPs remains unclear. To explore its role in the regulation of the apoptosis of insect cells, we constructed the transient expression vector (pIE1 vectors) and the recombinant baculovirus expressing Bsiap genes (from the Buzura suppressaria nucleopolyhedrovirus) of the group II baculovirus. Apoptosis gene expression results and the virus-induced apoptosis rate show that the overexpression of BsIAP1 could promote apoptosis in insect cells. However, the overexpression of BsIAP2 and BsIAP3 decreases the expression of apoptotic genes, revealing an inhibitory effect. Results on the impact of baculovirus-induced apoptosis also confirm that BsIAP1 reduces viral nucleocapsid expression and the baculovirus titer, while BsIAP2 and BsIAP3 increase them significantly. Furthermore, compared with single expression, the co-expression of BsIAP2 and BsIAP3 significantly reduces the rate of virus-induced apoptosis and improves the expression of nucleocapsids and the titer of offspring virus, indicating the synergistic effect on BsIAP2 and BsIAP3. In addition, combined expression of all three BsIAPs significantly reduced levels of intracellular apoptosis-related genes (including apoptosis and anti-apoptosis genes), as well as apoptosis rate and progeny virus titer, indicating that life activities in insect cells are also inhibited. These findings reveal the relationship between apoptosis and group II baculovirus IAP, which provide an experimental and theoretical basis for further exploration of the molecular mechanism between group II baculoviruses and insect cells. View Full-Text
Keywords: insect cell; group II baculovirus; inhibitor of apoptosis protein; apoptosis insect cell; group II baculovirus; inhibitor of apoptosis protein; apoptosis
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MDPI and ACS Style

Zheng, H.; Pan, Y.; Awais, M.M.; Tian, W.; Li, J.; Sun, J. Impact of Group II Baculovirus IAPs on Virus-Induced Apoptosis in Insect Cells. Genes 2022, 13, 750. https://doi.org/10.3390/genes13050750

AMA Style

Zheng H, Pan Y, Awais MM, Tian W, Li J, Sun J. Impact of Group II Baculovirus IAPs on Virus-Induced Apoptosis in Insect Cells. Genes. 2022; 13(5):750. https://doi.org/10.3390/genes13050750

Chicago/Turabian Style

Zheng, Hao, Yong Pan, Mian Muhammad Awais, Weibin Tian, Jingyang Li, and Jingchen Sun. 2022. "Impact of Group II Baculovirus IAPs on Virus-Induced Apoptosis in Insect Cells" Genes 13, no. 5: 750. https://doi.org/10.3390/genes13050750

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