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Correction published on 28 December 2021, see Genes 2022, 13(1), 69.
Article

Genome-Wide Analysis of Terpene Synthase Gene Family in Mentha longifolia and Catalytic Activity Analysis of a Single Terpene Synthase

1
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences (Nanjing Botanical Garden Mem. Sun Yat-Sen), Nanjing 210014, China
2
Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA
3
Zhejiang Provincial Key Laboratory of Resources Protection and Innovation of Traditional Chinese Medicine, Hangzhou 311300, China
4
Nanjing Foreign Language School, Nanjing 210008, China
5
Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Nanjing 210014, China
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College of Forest, Nanjing Forestry University, Nanjing 210037, China
7
Institute of Biological Chemistry and M.J. Murdock Metabolomics Laboratory, Washington State University, Pullman, WA 99164, USA
*
Authors to whom correspondence should be addressed.
Academic Editors: Elizabeth R. Waters, Tanya Renner and Bernd Weisshaar
Genes 2021, 12(4), 518; https://doi.org/10.3390/genes12040518
Received: 14 December 2020 / Revised: 30 March 2021 / Accepted: 31 March 2021 / Published: 2 April 2021 / Corrected: 28 December 2021
(This article belongs to the Special Issue Molecular Evolutionary and Comparative Genomics Analyses in Plants)
Terpenoids are a wide variety of natural products and terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids. Mentha plants are rich in essential oils, whose main components are terpenoids, and their biosynthetic pathways have been basically elucidated. However, there is a lack of systematic identification and study of TPS in Mentha plants. In this work, we genome-widely identified and analyzed the TPS gene family in Mentha longifolia, a model plant for functional genomic research in the genus Mentha. A total of 63 TPS genes were identified in the M. longifolia genome sequence assembly, which could be divided into six subfamilies. The TPS-b subfamily had the largest number of genes, which might be related to the abundant monoterpenoids in Mentha plants. The TPS-e subfamily had 18 members and showed a significant species-specific expansion compared with other sequenced Lamiaceae plant species. The 63 TPS genes could be mapped to nine scaffolds of the M. longifolia genome sequence assembly and the distribution of these genes is uneven. Tandem duplicates and fragment duplicates contributed greatly to the increase in the number of TPS genes in M. longifolia. The conserved motifs (RR(X)8W, NSE/DTE, RXR, and DDXXD) were analyzed in M. longifolia TPSs, and significant differentiation was found between different subfamilies. Adaptive evolution analysis showed that M. longifolia TPSs were subjected to purifying selection after the species-specific expansion, and some amino acid residues under positive selection were identified. Furthermore, we also cloned and analyzed the catalytic activity of a single terpene synthase, MlongTPS29, which belongs to the TPS-b subfamily. MlongTPS29 could encode a limonene synthase and catalyze the biosynthesis of limonene, an important precursor of essential oils from the genus Mentha. This study provides useful information for the biosynthesis of terpenoids in the genus Mentha. View Full-Text
Keywords: Mentha longifolia; terpene synthase; terpenoids; limonene synthase Mentha longifolia; terpene synthase; terpenoids; limonene synthase
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MDPI and ACS Style

Chen, Z.; Vining, K.J.; Qi, X.; Yu, X.; Zheng, Y.; Liu, Z.; Fang, H.; Li, L.; Bai, Y.; Liang, C.; Li, W.; Lange, B.M. Genome-Wide Analysis of Terpene Synthase Gene Family in Mentha longifolia and Catalytic Activity Analysis of a Single Terpene Synthase. Genes 2021, 12, 518. https://doi.org/10.3390/genes12040518

AMA Style

Chen Z, Vining KJ, Qi X, Yu X, Zheng Y, Liu Z, Fang H, Li L, Bai Y, Liang C, Li W, Lange BM. Genome-Wide Analysis of Terpene Synthase Gene Family in Mentha longifolia and Catalytic Activity Analysis of a Single Terpene Synthase. Genes. 2021; 12(4):518. https://doi.org/10.3390/genes12040518

Chicago/Turabian Style

Chen, Zequn, Kelly J. Vining, Xiwu Qi, Xu Yu, Ying Zheng, Zhiqi Liu, Hailing Fang, Li Li, Yang Bai, Chengyuan Liang, Weilin Li, and Bernd Markus Lange. 2021. "Genome-Wide Analysis of Terpene Synthase Gene Family in Mentha longifolia and Catalytic Activity Analysis of a Single Terpene Synthase" Genes 12, no. 4: 518. https://doi.org/10.3390/genes12040518

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