Next Article in Journal
Negative Regulation of ULK1 by microRNA-106a in Autophagy Induced by a Triple Drug Combination in Colorectal Cancer Cells In Vitro
Previous Article in Journal
Hybridogenesis in the Water Frogs from Western Russian Territory: Intrapopulation Variation in Genome Elimination
Article

Genomic Characterization Provides an Insight into the Pathogenicity of the Poplar Canker Bacterium Lonsdalea populi

Beijing Key Laboratory for Forest Pest Control, College of Forestry, Beijing Forestry University, Beijing 100083, China
*
Author to whom correspondence should be addressed.
Academic Editor: Anke Becker
Genes 2021, 12(2), 246; https://doi.org/10.3390/genes12020246
Received: 18 January 2021 / Revised: 28 January 2021 / Accepted: 4 February 2021 / Published: 9 February 2021
(This article belongs to the Section Molecular Genetics and Genomics)
An emerging poplar canker caused by the gram-negative bacterium, Lonsdalea populi, has led to high mortality of hybrid poplars Populus × euramericana in China and Europe. The molecular bases of pathogenicity and bark adaptation of L. populi have become a focus of recent research. This study revealed the whole genome sequence and identified putative virulence factors of L. populi. A high-quality L. populi genome sequence was assembled de novo, with a genome size of 3,859,707 bp, containing approximately 3434 genes and 107 RNAs (75 tRNA, 22 rRNA, and 10 ncRNA). The L. populi genome contained 380 virulence-associated genes, mainly encoding for adhesion, extracellular enzymes, secretory systems, and two-component transduction systems. The genome had 110 carbohydrate-active enzyme (CAZy)-coding genes and putative secreted proteins. The antibiotic-resistance database annotation listed that L. populi was resistant to penicillin, fluoroquinolone, and kasugamycin. Analysis of comparative genomics found that L. populi exhibited the highest homology with the L. britannica genome and L. populi encompassed 1905 specific genes, 1769 dispensable genes, and 1381 conserved genes, suggesting high evolutionary diversity and genomic plasticity. Moreover, the pan genome analysis revealed that the N-5-1 genome is an open genome. These findings provide important resources for understanding the molecular basis of the pathogenicity and biology of L. populi and the poplar-bacterium interaction. View Full-Text
Keywords: poplar canker; Lonsdalea populi; genome; pathogenicity; virulence genes poplar canker; Lonsdalea populi; genome; pathogenicity; virulence genes
Show Figures

Figure 1

MDPI and ACS Style

Chen, X.; Li, R.; Wang, Y.; Li, A. Genomic Characterization Provides an Insight into the Pathogenicity of the Poplar Canker Bacterium Lonsdalea populi. Genes 2021, 12, 246. https://doi.org/10.3390/genes12020246

AMA Style

Chen X, Li R, Wang Y, Li A. Genomic Characterization Provides an Insight into the Pathogenicity of the Poplar Canker Bacterium Lonsdalea populi. Genes. 2021; 12(2):246. https://doi.org/10.3390/genes12020246

Chicago/Turabian Style

Chen, Xiaomeng, Rui Li, Yonglin Wang, and Aining Li. 2021. "Genomic Characterization Provides an Insight into the Pathogenicity of the Poplar Canker Bacterium Lonsdalea populi" Genes 12, no. 2: 246. https://doi.org/10.3390/genes12020246

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop